Melanin nanoparticles enhance the neuroprotection of mesenchymal stem cells against hypoxic‐ischemic injury by inhibiting apoptosis and upregulating antioxidant defense

Polydopamine nanoparticles are artificial melanin nanoparticles (MNPs) that show strong antioxidant activity. The effects of MNPs on the neuroprotection of mesenchymal stem cells (MSCs) against hypoxic‐ischemic injury and the underlying mechanism have not yet been revealed. In this study, an oxygen–...

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Bibliographic Details
Published in:Cell biology international 2022-06, Vol.46 (6), p.933-946
Main Authors: Tang, Chunliu, Luo, Jiefeng, Yan, Xianjia, Huang, Qiaojuan, Huang, Zhenhua, Luo, Qi, Lan, Yuan, Chen, Dingzhi, Zhang, Baolin, Chen, Menghua, Kong, Deyan
Format: Article
Language:English
Subjects:
antioxidant
Antioxidants
Apoptosis
BAX protein
Caspase
Hypoxia
Ischemia
ischemic stroke
Melanin
melanin nanoparticles
Mesenchymal stem cells
Nanoparticles
Neuroprotection
Reactive oxygen species
Stem cells
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Summary:Polydopamine nanoparticles are artificial melanin nanoparticles (MNPs) that show strong antioxidant activity. The effects of MNPs on the neuroprotection of mesenchymal stem cells (MSCs) against hypoxic‐ischemic injury and the underlying mechanism have not yet been revealed. In this study, an oxygen–glucose deprivation (OGD)‐injured neuron model was used to mimic neuronal hypoxic‐ischemic injury in vitro. MSCs pretreated with MNPs and then cocultured with OGD‐injured neurons were used to investigate the potential effects of MNPs on the neuroprotection of MSCs and to elucidate the underlying mechanism. After coculturing with MNPs‐pretreated MSCs, MSCs, and MNPs in a transwell coculture system, the OGD‐injured neurons were rescued by 91.24%, 79.32%, and 59.97%, respectively. Further data demonstrated that MNPs enhanced the neuroprotection against hypoxic‐ischemic injury of MSCs by scavenging reactive oxygen species and superoxide and attenuating neuronal apoptosis by deactivating caspase‐3, downregulating the expression of proapoptotic Bax proteins, and upregulating the expression of antiapoptotic Bcl‐2 proteins. These findings suggest that MNPs enhance the neuroprotective effect of MSCs against hypoxic‐ischemic injury by inhibiting apoptosis and upregulating antioxidant defense, which could provide some evidence for the potential application of combined MNPs and MSCs in the therapy for ischemic stroke.
ISSN:1065-6995
1095-8355
DOI:10.1002/cbin.11781