Simultaneous quantification of six indicator compounds in Wen-Qing-Yin by high-performance liquid chromatography-diode array detection

A simple gradient high-performance liquid chromatography with diode array detection (HPLC-DAD) method was used to simultaneously to analyze characteristics of six indicator compounds in the traditional Chinese medicine (TCM) formulation Wen-Qing-Yin (WQY). Separate optimization was performed using a...

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Bibliographic Details
Published in:Yàowu shi͡p︡in fenxi 2019-07, Vol.27 (3), p.749-757
Main Authors: Yeh, Chi-Chen, Huang, Shih-Shan, Liu, Po-Yu, Wang, Bo-Cheng, Tsai, Chia-Fen, Wang, Der-Yuan, Cheng, Hwei-Fang
Format: Article
Language:English
Subjects:
Acetonitrile
Arrays
Baicalin
Berberine
Calibration
Chinese medicine
Chromatography
Correlation coefficient
Correlation coefficients
Ferulic acid
Flow velocity
Formic acid
Herbal medicine
High performance liquid chromatography
HPLC-DAD
Indicators
Liquid chromatography
Method validation
Methods
Optimization
Original
PCA analysis
Principal components analysis
Qualitative analysis
Quality control
Quality standards
Recovery
Regression analysis
Traditional Chinese medicine
Wen-Qing-Yin
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Summary:A simple gradient high-performance liquid chromatography with diode array detection (HPLC-DAD) method was used to simultaneously to analyze characteristics of six indicator compounds in the traditional Chinese medicine (TCM) formulation Wen-Qing-Yin (WQY). Separate optimization was performed using a Cosmosil C18 column gradient method with 0.1% formic acid in both mobile phases of aqueous and acetonitrile (ACN), at a flow rate, detection wavelength, and sample volume of 1.8 mL/min, 268 nm, and 10 μL, respectively. The linear regression of six active compounds berberine (BER), baicalin (BAI), ferulic acid (FER), geniposide (GEN), hydorxymethoxylfurfural (HMF), and paeoniflorin (PAE) was produced at the concentration range of 10–2000 μg/mL. The method validation revealed an acceptable precision (intra- and inter-day precision 
ISSN:1021-9498
2224-6614
DOI:10.1016/j.jfda.2018.12.003