Inhibitory Effect of miR-339-5p on Glioma through PTP4A1/HMGB1 Pathway

Objective. Finding miR-339-5p inhibitory functions in glioma through PTP4A1/HMGB1 pathway. Methods. From May 2020 to August 2021, 20 glioblastoma and para cancer tissues were chosen for qRT-PCR analysis. The miR-NC, miR-con, miR-339-5PMIMIC, and miR-con + groups were transfected into human glioma U2...

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Bibliographic Details
Published in:Disease markers 2022-07, Vol.2022, p.1-8
Main Authors: Zheng, Buyi, Wang, Shouyi, Shen, Huanan, Lin, Jie
Format: Article
Language:English
Subjects:
Angiogenesis
Brain cancer
Brain research
Cancer
Cell growth
Cell migration
Genes
Glioblastoma
Glioma cells
HMGB1 protein
Invasiveness
MicroRNAs
Mimicry
Proteins
Reporter gene
Scratching
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Summary:Objective. Finding miR-339-5p inhibitory functions in glioma through PTP4A1/HMGB1 pathway. Methods. From May 2020 to August 2021, 20 glioblastoma and para cancer tissues were chosen for qRT-PCR analysis. The miR-NC, miR-con, miR-339-5PMIMIC, and miR-con + groups were transfected into human glioma U251 cells. The capacity of cell vascular-like structure construction was found by simulating angiogenesis, and the ability of cell movement was examined by cell scratching. The twofold luciferase reporter gene method determined that miR-339-5p targets PTP4A1, and the protein expression levels of PTP4A1 and HMGB1 were examined using Western blot. Results. MiR-339-5P expression was substantially lower in cancer samples than noncancer samples (P
ISSN:0278-0240
1875-8630
DOI:10.1155/2022/2231195