Inhibitory Effect of miR-339-5p on Glioma through PTP4A1/HMGB1 Pathway

Objective. Finding miR-339-5p inhibitory functions in glioma through PTP4A1/HMGB1 pathway. Methods. From May 2020 to August 2021, 20 glioblastoma and para cancer tissues were chosen for qRT-PCR analysis. The miR-NC, miR-con, miR-339-5PMIMIC, and miR-con + groups were transfected into human glioma U2...

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Published in:Disease markers 2022-07, Vol.2022, p.1-8
Main Authors: Zheng, Buyi, Wang, Shouyi, Shen, Huanan, Lin, Jie
Format: Article
Language:English
Subjects:
Angiogenesis
Brain cancer
Brain research
Cancer
Cell growth
Cell migration
Genes
Glioblastoma
Glioma cells
HMGB1 protein
Invasiveness
MicroRNAs
Mimicry
Proteins
Reporter gene
Scratching
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creator Zheng, Buyi
Wang, Shouyi
Shen, Huanan
Lin, Jie
description Objective. Finding miR-339-5p inhibitory functions in glioma through PTP4A1/HMGB1 pathway. Methods. From May 2020 to August 2021, 20 glioblastoma and para cancer tissues were chosen for qRT-PCR analysis. The miR-NC, miR-con, miR-339-5PMIMIC, and miR-con + groups were transfected into human glioma U251 cells. The capacity of cell vascular-like structure construction was found by simulating angiogenesis, and the ability of cell movement was examined by cell scratching. The twofold luciferase reporter gene method determined that miR-339-5p targets PTP4A1, and the protein expression levels of PTP4A1 and HMGB1 were examined using Western blot. Results. MiR-339-5P expression was substantially lower in cancer samples than noncancer samples (P
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Finding miR-339-5p inhibitory functions in glioma through PTP4A1/HMGB1 pathway. Methods. From May 2020 to August 2021, 20 glioblastoma and para cancer tissues were chosen for qRT-PCR analysis. The miR-NC, miR-con, miR-339-5PMIMIC, and miR-con + groups were transfected into human glioma U251 cells. The capacity of cell vascular-like structure construction was found by simulating angiogenesis, and the ability of cell movement was examined by cell scratching. The twofold luciferase reporter gene method determined that miR-339-5p targets PTP4A1, and the protein expression levels of PTP4A1 and HMGB1 were examined using Western blot. Results. MiR-339-5P expression was substantially lower in cancer samples than noncancer samples (P<0.05). PTP4A1 expression in cancer samples was higher than in healthy controls (P<0.05). The miR-339-5p group produced significantly less vascular-like structures than the NC and miR-con groups (P<0.05). The miR-339-5p group lowered the invasive index and migratory rate of U251 cells (P<0.05). PTP4A1 inhibited the luciferase activity of the pTP4A1-WT reporter gene (P<0.05) but not the PTP4A1-MUT (P>0.05). The miR-339-5p group had lower protein levels of PTP4A1 and HMGB1 than the NC and miR-con groups (P<0.05). The development of vascular-like structures was substantially more significant in the miR-con +PTP4A1 group than in the miR-con and miR-339-5p +PTP4A1 groups (P<0.05). In terms of migration and invasion index, there was a substantial difference between the miR-339-5p +PTP4A1 and the miR-con +PTP4A1 groups (P<0.05). The miR-con +PTP4A1 group had a greater migration rate and invasive index than the miR-con and miR-339-5p +PTP4A1 groups (P<0.05). Conclusion. MiR-339-5P inhibits angiogenic mimicry, migration, and invasion of brain glioma U251 cells by inhibiting the PTP4A1/HMGB1 signal pathway.]]></description><identifier>ISSN: 0278-0240</identifier><identifier>EISSN: 1875-8630</identifier><identifier>DOI: 10.1155/2022/2231195</identifier><identifier>PMID: 35872698</identifier><language>eng</language><publisher>Amsterdam: Hindawi</publisher><subject>Angiogenesis ; Brain cancer ; Brain research ; Cancer ; Cell growth ; Cell migration ; Genes ; Glioblastoma ; Glioma cells ; HMGB1 protein ; Invasiveness ; MicroRNAs ; Mimicry ; Proteins ; Reporter gene ; Scratching</subject><ispartof>Disease markers, 2022-07, Vol.2022, p.1-8</ispartof><rights>Copyright © 2022 Buyi Zheng et al.</rights><rights>Copyright © 2022 Buyi Zheng et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2022 Buyi Zheng et al. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-cbe42e371129449c1823aff573ccfaed543fda759181a66a3abb752e1f0d2a1b3</citedby><cites>FETCH-LOGICAL-c425t-cbe42e371129449c1823aff573ccfaed543fda759181a66a3abb752e1f0d2a1b3</cites><orcidid>0000-0001-8748-2820 ; 0000-0003-1755-3624 ; 0000-0002-7221-4134 ; 0000-0002-4192-773X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids></links><search><contributor>Bukhari, Ihtisham</contributor><contributor>Ihtisham Bukhari</contributor><creatorcontrib>Zheng, Buyi</creatorcontrib><creatorcontrib>Wang, Shouyi</creatorcontrib><creatorcontrib>Shen, Huanan</creatorcontrib><creatorcontrib>Lin, Jie</creatorcontrib><title>Inhibitory Effect of miR-339-5p on Glioma through PTP4A1/HMGB1 Pathway</title><title>Disease markers</title><description><![CDATA[Objective. Finding miR-339-5p inhibitory functions in glioma through PTP4A1/HMGB1 pathway. Methods. From May 2020 to August 2021, 20 glioblastoma and para cancer tissues were chosen for qRT-PCR analysis. The miR-NC, miR-con, miR-339-5PMIMIC, and miR-con + groups were transfected into human glioma U251 cells. The capacity of cell vascular-like structure construction was found by simulating angiogenesis, and the ability of cell movement was examined by cell scratching. The twofold luciferase reporter gene method determined that miR-339-5p targets PTP4A1, and the protein expression levels of PTP4A1 and HMGB1 were examined using Western blot. Results. MiR-339-5P expression was substantially lower in cancer samples than noncancer samples (P<0.05). PTP4A1 expression in cancer samples was higher than in healthy controls (P<0.05). The miR-339-5p group produced significantly less vascular-like structures than the NC and miR-con groups (P<0.05). The miR-339-5p group lowered the invasive index and migratory rate of U251 cells (P<0.05). PTP4A1 inhibited the luciferase activity of the pTP4A1-WT reporter gene (P<0.05) but not the PTP4A1-MUT (P>0.05). The miR-339-5p group had lower protein levels of PTP4A1 and HMGB1 than the NC and miR-con groups (P<0.05). The development of vascular-like structures was substantially more significant in the miR-con +PTP4A1 group than in the miR-con and miR-339-5p +PTP4A1 groups (P<0.05). In terms of migration and invasion index, there was a substantial difference between the miR-339-5p +PTP4A1 and the miR-con +PTP4A1 groups (P<0.05). The miR-con +PTP4A1 group had a greater migration rate and invasive index than the miR-con and miR-339-5p +PTP4A1 groups (P<0.05). Conclusion. MiR-339-5P inhibits angiogenic mimicry, migration, and invasion of brain glioma U251 cells by inhibiting the PTP4A1/HMGB1 signal pathway.]]></description><subject>Angiogenesis</subject><subject>Brain cancer</subject><subject>Brain research</subject><subject>Cancer</subject><subject>Cell growth</subject><subject>Cell migration</subject><subject>Genes</subject><subject>Glioblastoma</subject><subject>Glioma cells</subject><subject>HMGB1 protein</subject><subject>Invasiveness</subject><subject>MicroRNAs</subject><subject>Mimicry</subject><subject>Proteins</subject><subject>Reporter gene</subject><subject>Scratching</subject><issn>0278-0240</issn><issn>1875-8630</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp90U1L7DAUBuAgis5Vd_6Aghvh3jo5-ZgkG8ErOgqKg-g6pGliI20zpq0y_94OMwj3LlxlcR5ezsmL0AngcwDOpwQTMiWEAii-gyYgBc_ljOJdNMFEyBwThg_Qr657wxiIYmofHVAuBZkpOUE3d20VitDHtMquvXe2z6LPmvCUU6pyvsxim83rEBuT9VWKw2uVLZ4X7BKmtw_zv5AtTF99mtUR2vOm7tzx9j1ELzfXz1e3-f3j_O7q8j63jPA-t4VjxFEB60WYsiAJNd5zQa31xpWcUV8awRVIMLOZoaYoBCcOPC6JgYIeootN7nIoGlda1_bJ1HqZQmPSSkcT9L-TNlT6NX5oRbGgko4BZ9uAFN8H1_W6CZ11dW1aF4dOj7_CGDAJbKSn_9G3OKR2PG-tKFdKChjVn42yKXZdcv57GcB6XZBeF6S3BY3894ZXoS3NZ_hZfwGVb4ty</recordid><startdate>20220715</startdate><enddate>20220715</enddate><creator>Zheng, Buyi</creator><creator>Wang, Shouyi</creator><creator>Shen, Huanan</creator><creator>Lin, Jie</creator><general>Hindawi</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8748-2820</orcidid><orcidid>https://orcid.org/0000-0003-1755-3624</orcidid><orcidid>https://orcid.org/0000-0002-7221-4134</orcidid><orcidid>https://orcid.org/0000-0002-4192-773X</orcidid></search><sort><creationdate>20220715</creationdate><title>Inhibitory Effect of miR-339-5p on Glioma through PTP4A1/HMGB1 Pathway</title><author>Zheng, Buyi ; Wang, Shouyi ; Shen, Huanan ; Lin, Jie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-cbe42e371129449c1823aff573ccfaed543fda759181a66a3abb752e1f0d2a1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Angiogenesis</topic><topic>Brain cancer</topic><topic>Brain research</topic><topic>Cancer</topic><topic>Cell growth</topic><topic>Cell migration</topic><topic>Genes</topic><topic>Glioblastoma</topic><topic>Glioma cells</topic><topic>HMGB1 protein</topic><topic>Invasiveness</topic><topic>MicroRNAs</topic><topic>Mimicry</topic><topic>Proteins</topic><topic>Reporter gene</topic><topic>Scratching</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Buyi</creatorcontrib><creatorcontrib>Wang, Shouyi</creatorcontrib><creatorcontrib>Shen, Huanan</creatorcontrib><creatorcontrib>Lin, Jie</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Disease markers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Buyi</au><au>Wang, Shouyi</au><au>Shen, Huanan</au><au>Lin, Jie</au><au>Bukhari, Ihtisham</au><au>Ihtisham Bukhari</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory Effect of miR-339-5p on Glioma through PTP4A1/HMGB1 Pathway</atitle><jtitle>Disease markers</jtitle><date>2022-07-15</date><risdate>2022</risdate><volume>2022</volume><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>0278-0240</issn><eissn>1875-8630</eissn><abstract><![CDATA[Objective. Finding miR-339-5p inhibitory functions in glioma through PTP4A1/HMGB1 pathway. Methods. From May 2020 to August 2021, 20 glioblastoma and para cancer tissues were chosen for qRT-PCR analysis. The miR-NC, miR-con, miR-339-5PMIMIC, and miR-con + groups were transfected into human glioma U251 cells. The capacity of cell vascular-like structure construction was found by simulating angiogenesis, and the ability of cell movement was examined by cell scratching. The twofold luciferase reporter gene method determined that miR-339-5p targets PTP4A1, and the protein expression levels of PTP4A1 and HMGB1 were examined using Western blot. Results. MiR-339-5P expression was substantially lower in cancer samples than noncancer samples (P<0.05). PTP4A1 expression in cancer samples was higher than in healthy controls (P<0.05). The miR-339-5p group produced significantly less vascular-like structures than the NC and miR-con groups (P<0.05). The miR-339-5p group lowered the invasive index and migratory rate of U251 cells (P<0.05). PTP4A1 inhibited the luciferase activity of the pTP4A1-WT reporter gene (P<0.05) but not the PTP4A1-MUT (P>0.05). The miR-339-5p group had lower protein levels of PTP4A1 and HMGB1 than the NC and miR-con groups (P<0.05). The development of vascular-like structures was substantially more significant in the miR-con +PTP4A1 group than in the miR-con and miR-339-5p +PTP4A1 groups (P<0.05). In terms of migration and invasion index, there was a substantial difference between the miR-339-5p +PTP4A1 and the miR-con +PTP4A1 groups (P<0.05). The miR-con +PTP4A1 group had a greater migration rate and invasive index than the miR-con and miR-339-5p +PTP4A1 groups (P<0.05). Conclusion. MiR-339-5P inhibits angiogenic mimicry, migration, and invasion of brain glioma U251 cells by inhibiting the PTP4A1/HMGB1 signal pathway.]]></abstract><cop>Amsterdam</cop><pub>Hindawi</pub><pmid>35872698</pmid><doi>10.1155/2022/2231195</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-8748-2820</orcidid><orcidid>https://orcid.org/0000-0003-1755-3624</orcidid><orcidid>https://orcid.org/0000-0002-7221-4134</orcidid><orcidid>https://orcid.org/0000-0002-4192-773X</orcidid><oa>free_for_read</oa></addata></record>
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subjects Angiogenesis
Brain cancer
Brain research
Cancer
Cell growth
Cell migration
Genes
Glioblastoma
Glioma cells
HMGB1 protein
Invasiveness
MicroRNAs
Mimicry
Proteins
Reporter gene
Scratching
title Inhibitory Effect of miR-339-5p on Glioma through PTP4A1/HMGB1 Pathway
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