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A carvacrol‐based product reduces Campylobacter jejuni load and alters microbiota composition in the caeca of chickens
Aim This study was conducted to test the ability of a carvacrol‐based formulation (Phodé, France) to decrease the C. jejuni caecal load in inoculated broiler chickens and to study the impact of the C. jejuni inoculation alone or combined with the product, on the caecal microbiota. Methods and Result...
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| Published in: | Journal of applied microbiology 2022-06, Vol.132 (6), p.4501-4516 |
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| Main Authors: | , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c4771-a5e45cb840d38f02f0ca76a1c5e0a5bb67b515328e7982f71ee9d551edea8f8e3 |
| container_end_page | 4516 |
| container_issue | 6 |
| container_start_page | 4501 |
| container_title | Journal of applied microbiology |
| container_volume | 132 |
| creator | Allaoua, Marion Bonnafé, Elsa Etienne, Pierre Noirot, Virginie Gabarrou, Jean‐François Castinel, Adrien Pascal, Géraldine Darbot, Vincent Treilhou, Michel Combes, Sylvie |
| description | Aim
This study was conducted to test the ability of a carvacrol‐based formulation (Phodé, France) to decrease the C. jejuni caecal load in inoculated broiler chickens and to study the impact of the C. jejuni inoculation alone or combined with the product, on the caecal microbiota.
Methods and Results
On day 1, chickens were either fed a control feed or the same diet supplemented with a carvacrol‐based product. On day 21, the carvacrol‐supplemented chickens and half of the non‐supplemented chickens were inoculated with C. jejuni (108 CFU). Quantitative PCR was used to quantify C. jejuni in chicken caecal samples and 16S rRNA gene sequencing was carried out at 25, 31 and 35 days of age. A significant decrease of 1.4 log of the C. jejuni caecal load was observed in 35‐day‐old chickens supplemented with the product, compared to the inoculated and unsupplemented group (p |
| doi_str_mv | 10.1111/jam.15521 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9314584</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2638748034</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4771-a5e45cb840d38f02f0ca76a1c5e0a5bb67b515328e7982f71ee9d551edea8f8e3</originalsourceid><addsrcrecordid>eNp1kc1u1DAQxyMEoh9w4AWQJS70kNYfcey9IK1W0IIWcYGzNXEmrEMSBztZ2BuPwDPyJPV2S1UqYckaa_yf39jzz7IXjJ6ztC5a6M-ZlJw9yo6ZKGXOS8Uf35yLXFLFj7KTGFtKmaCyfJodCcmVpkwdZz-XxELYgg2--_PrdwURazIGX892IgFTwEhW0I-7zldgJwykxXYeHOk81ASGtLuUjaR3iVE5PwGxvh99dJPzA3EDmTaYmqAF4htiN85-wyE-y5400EV8fhtPsy_v3n5eXeXrT5fvV8t1bgulWA4SC2krXdBa6IbyhlpQJTArkYKsqlJVkknBNaqF5o1iiItaSoY1gm40itPszYE7zlWPtcVhCtCZMbgews54cObfm8FtzFe_NQvBCqmLBDg7ADYPyq6Wa7PPUVFSJbjcsqR9fdss-O8zxsn0LlrsOhjQz9HwUmhVaCr22FcPpK2fw5BGkVRlyfWC0nvN02xjDNjcvYBRs_feJO_NjfdJ-_L-T--Uf81OgouD4IfrcPd_kvmw_HhAXgPVv7ru</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2666289004</pqid></control><display><type>article</type><title>A carvacrol‐based product reduces Campylobacter jejuni load and alters microbiota composition in the caeca of chickens</title><source>Alma/SFX Local Collection</source><creator>Allaoua, Marion ; Bonnafé, Elsa ; Etienne, Pierre ; Noirot, Virginie ; Gabarrou, Jean‐François ; Castinel, Adrien ; Pascal, Géraldine ; Darbot, Vincent ; Treilhou, Michel ; Combes, Sylvie</creator><creatorcontrib>Allaoua, Marion ; Bonnafé, Elsa ; Etienne, Pierre ; Noirot, Virginie ; Gabarrou, Jean‐François ; Castinel, Adrien ; Pascal, Géraldine ; Darbot, Vincent ; Treilhou, Michel ; Combes, Sylvie</creatorcontrib><description>Aim
This study was conducted to test the ability of a carvacrol‐based formulation (Phodé, France) to decrease the C. jejuni caecal load in inoculated broiler chickens and to study the impact of the C. jejuni inoculation alone or combined with the product, on the caecal microbiota.
Methods and Results
On day 1, chickens were either fed a control feed or the same diet supplemented with a carvacrol‐based product. On day 21, the carvacrol‐supplemented chickens and half of the non‐supplemented chickens were inoculated with C. jejuni (108 CFU). Quantitative PCR was used to quantify C. jejuni in chicken caecal samples and 16S rRNA gene sequencing was carried out at 25, 31 and 35 days of age. A significant decrease of 1.4 log of the C. jejuni caecal load was observed in 35‐day‐old chickens supplemented with the product, compared to the inoculated and unsupplemented group (p < 0.05). The inoculation with C. jejuni significantly increased the population richness, Shannon and Simpson diversity and altered beta‐diversity. Compared to the control group, the C. jejuni inoculation causes significant changes in the microbiota. The carvacrol‐based product associated with C. jejuni inoculation increased the diversity and strongly modified the structure of the microbial community. Functional analysis by 16S rRNA gene‐based predictions further revealed that the product up‐regulated the pathways involved in the antimicrobial synthesis, which could explain its shaping effect on the caecal microbiota.
Conclusions
Our study confirmed the impairment of the caecal bacterial community after inoculation and demonstrated the ability of the product to reduce the C. jejuni load in chickens. Further investigations are needed to better understand the mode of action of this product to promote the installation of a beneficial microbiota to its host.
Significance and Impact of the Study
Results suggested that this product could be promising to control C. jejuni contamination of broilers.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.15521</identifier><identifier>PMID: 35278017</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>16Sr RNA gene sequencing ; Animal biology ; Antiinfectives and antibacterials ; Bacteriology ; Biotechnology ; broiler chicken ; Campylobacter ; Campylobacter jejuni ; Carvacrol ; Chickens ; essential oil ; feed additive ; Food and Nutrition ; foodborne disease ; Functional analysis ; Gene sequencing ; Inoculation ; Life Sciences ; Microbiology and Parasitology ; microbiome ; Microbiota ; Microorganisms ; Mode of action ; Original ; PICRUSt2 ; Pollution control ; Poultry ; rRNA 16S ; Veterinary medicine and animal Health</subject><ispartof>Journal of applied microbiology, 2022-06, Vol.132 (6), p.4501-4516</ispartof><rights>2022 Phodé. published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.</rights><rights>2022 Phodé. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.</rights><rights>2022. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Attribution - NonCommercial - NoDerivatives</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4771-a5e45cb840d38f02f0ca76a1c5e0a5bb67b515328e7982f71ee9d551edea8f8e3</citedby><cites>FETCH-LOGICAL-c4771-a5e45cb840d38f02f0ca76a1c5e0a5bb67b515328e7982f71ee9d551edea8f8e3</cites><orcidid>0000-0001-6366-2480 ; 0000-0001-7000-3408 ; 0000-0002-2945-4423</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35278017$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-03607325$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Allaoua, Marion</creatorcontrib><creatorcontrib>Bonnafé, Elsa</creatorcontrib><creatorcontrib>Etienne, Pierre</creatorcontrib><creatorcontrib>Noirot, Virginie</creatorcontrib><creatorcontrib>Gabarrou, Jean‐François</creatorcontrib><creatorcontrib>Castinel, Adrien</creatorcontrib><creatorcontrib>Pascal, Géraldine</creatorcontrib><creatorcontrib>Darbot, Vincent</creatorcontrib><creatorcontrib>Treilhou, Michel</creatorcontrib><creatorcontrib>Combes, Sylvie</creatorcontrib><title>A carvacrol‐based product reduces Campylobacter jejuni load and alters microbiota composition in the caeca of chickens</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aim
This study was conducted to test the ability of a carvacrol‐based formulation (Phodé, France) to decrease the C. jejuni caecal load in inoculated broiler chickens and to study the impact of the C. jejuni inoculation alone or combined with the product, on the caecal microbiota.
Methods and Results
On day 1, chickens were either fed a control feed or the same diet supplemented with a carvacrol‐based product. On day 21, the carvacrol‐supplemented chickens and half of the non‐supplemented chickens were inoculated with C. jejuni (108 CFU). Quantitative PCR was used to quantify C. jejuni in chicken caecal samples and 16S rRNA gene sequencing was carried out at 25, 31 and 35 days of age. A significant decrease of 1.4 log of the C. jejuni caecal load was observed in 35‐day‐old chickens supplemented with the product, compared to the inoculated and unsupplemented group (p < 0.05). The inoculation with C. jejuni significantly increased the population richness, Shannon and Simpson diversity and altered beta‐diversity. Compared to the control group, the C. jejuni inoculation causes significant changes in the microbiota. The carvacrol‐based product associated with C. jejuni inoculation increased the diversity and strongly modified the structure of the microbial community. Functional analysis by 16S rRNA gene‐based predictions further revealed that the product up‐regulated the pathways involved in the antimicrobial synthesis, which could explain its shaping effect on the caecal microbiota.
Conclusions
Our study confirmed the impairment of the caecal bacterial community after inoculation and demonstrated the ability of the product to reduce the C. jejuni load in chickens. Further investigations are needed to better understand the mode of action of this product to promote the installation of a beneficial microbiota to its host.
Significance and Impact of the Study
Results suggested that this product could be promising to control C. jejuni contamination of broilers.</description><subject>16Sr RNA gene sequencing</subject><subject>Animal biology</subject><subject>Antiinfectives and antibacterials</subject><subject>Bacteriology</subject><subject>Biotechnology</subject><subject>broiler chicken</subject><subject>Campylobacter</subject><subject>Campylobacter jejuni</subject><subject>Carvacrol</subject><subject>Chickens</subject><subject>essential oil</subject><subject>feed additive</subject><subject>Food and Nutrition</subject><subject>foodborne disease</subject><subject>Functional analysis</subject><subject>Gene sequencing</subject><subject>Inoculation</subject><subject>Life Sciences</subject><subject>Microbiology and Parasitology</subject><subject>microbiome</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Mode of action</subject><subject>Original</subject><subject>PICRUSt2</subject><subject>Pollution control</subject><subject>Poultry</subject><subject>rRNA 16S</subject><subject>Veterinary medicine and animal Health</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNp1kc1u1DAQxyMEoh9w4AWQJS70kNYfcey9IK1W0IIWcYGzNXEmrEMSBztZ2BuPwDPyJPV2S1UqYckaa_yf39jzz7IXjJ6ztC5a6M-ZlJw9yo6ZKGXOS8Uf35yLXFLFj7KTGFtKmaCyfJodCcmVpkwdZz-XxELYgg2--_PrdwURazIGX892IgFTwEhW0I-7zldgJwykxXYeHOk81ASGtLuUjaR3iVE5PwGxvh99dJPzA3EDmTaYmqAF4htiN85-wyE-y5400EV8fhtPsy_v3n5eXeXrT5fvV8t1bgulWA4SC2krXdBa6IbyhlpQJTArkYKsqlJVkknBNaqF5o1iiItaSoY1gm40itPszYE7zlWPtcVhCtCZMbgews54cObfm8FtzFe_NQvBCqmLBDg7ADYPyq6Wa7PPUVFSJbjcsqR9fdss-O8zxsn0LlrsOhjQz9HwUmhVaCr22FcPpK2fw5BGkVRlyfWC0nvN02xjDNjcvYBRs_feJO_NjfdJ-_L-T--Uf81OgouD4IfrcPd_kvmw_HhAXgPVv7ru</recordid><startdate>202206</startdate><enddate>202206</enddate><creator>Allaoua, Marion</creator><creator>Bonnafé, Elsa</creator><creator>Etienne, Pierre</creator><creator>Noirot, Virginie</creator><creator>Gabarrou, Jean‐François</creator><creator>Castinel, Adrien</creator><creator>Pascal, Géraldine</creator><creator>Darbot, Vincent</creator><creator>Treilhou, Michel</creator><creator>Combes, Sylvie</creator><general>Oxford University Press</general><general>Wiley</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6366-2480</orcidid><orcidid>https://orcid.org/0000-0001-7000-3408</orcidid><orcidid>https://orcid.org/0000-0002-2945-4423</orcidid></search><sort><creationdate>202206</creationdate><title>A carvacrol‐based product reduces Campylobacter jejuni load and alters microbiota composition in the caeca of chickens</title><author>Allaoua, Marion ; Bonnafé, Elsa ; Etienne, Pierre ; Noirot, Virginie ; Gabarrou, Jean‐François ; Castinel, Adrien ; Pascal, Géraldine ; Darbot, Vincent ; Treilhou, Michel ; Combes, Sylvie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4771-a5e45cb840d38f02f0ca76a1c5e0a5bb67b515328e7982f71ee9d551edea8f8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>16Sr RNA gene sequencing</topic><topic>Animal biology</topic><topic>Antiinfectives and antibacterials</topic><topic>Bacteriology</topic><topic>Biotechnology</topic><topic>broiler chicken</topic><topic>Campylobacter</topic><topic>Campylobacter jejuni</topic><topic>Carvacrol</topic><topic>Chickens</topic><topic>essential oil</topic><topic>feed additive</topic><topic>Food and Nutrition</topic><topic>foodborne disease</topic><topic>Functional analysis</topic><topic>Gene sequencing</topic><topic>Inoculation</topic><topic>Life Sciences</topic><topic>Microbiology and Parasitology</topic><topic>microbiome</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Mode of action</topic><topic>Original</topic><topic>PICRUSt2</topic><topic>Pollution control</topic><topic>Poultry</topic><topic>rRNA 16S</topic><topic>Veterinary medicine and animal Health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Allaoua, Marion</creatorcontrib><creatorcontrib>Bonnafé, Elsa</creatorcontrib><creatorcontrib>Etienne, Pierre</creatorcontrib><creatorcontrib>Noirot, Virginie</creatorcontrib><creatorcontrib>Gabarrou, Jean‐François</creatorcontrib><creatorcontrib>Castinel, Adrien</creatorcontrib><creatorcontrib>Pascal, Géraldine</creatorcontrib><creatorcontrib>Darbot, Vincent</creatorcontrib><creatorcontrib>Treilhou, Michel</creatorcontrib><creatorcontrib>Combes, Sylvie</creatorcontrib><collection>Wiley-Blackwell Open Access Collection</collection><collection>Wiley Free Archive</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Allaoua, Marion</au><au>Bonnafé, Elsa</au><au>Etienne, Pierre</au><au>Noirot, Virginie</au><au>Gabarrou, Jean‐François</au><au>Castinel, Adrien</au><au>Pascal, Géraldine</au><au>Darbot, Vincent</au><au>Treilhou, Michel</au><au>Combes, Sylvie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A carvacrol‐based product reduces Campylobacter jejuni load and alters microbiota composition in the caeca of chickens</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2022-06</date><risdate>2022</risdate><volume>132</volume><issue>6</issue><spage>4501</spage><epage>4516</epage><pages>4501-4516</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aim
This study was conducted to test the ability of a carvacrol‐based formulation (Phodé, France) to decrease the C. jejuni caecal load in inoculated broiler chickens and to study the impact of the C. jejuni inoculation alone or combined with the product, on the caecal microbiota.
Methods and Results
On day 1, chickens were either fed a control feed or the same diet supplemented with a carvacrol‐based product. On day 21, the carvacrol‐supplemented chickens and half of the non‐supplemented chickens were inoculated with C. jejuni (108 CFU). Quantitative PCR was used to quantify C. jejuni in chicken caecal samples and 16S rRNA gene sequencing was carried out at 25, 31 and 35 days of age. A significant decrease of 1.4 log of the C. jejuni caecal load was observed in 35‐day‐old chickens supplemented with the product, compared to the inoculated and unsupplemented group (p < 0.05). The inoculation with C. jejuni significantly increased the population richness, Shannon and Simpson diversity and altered beta‐diversity. Compared to the control group, the C. jejuni inoculation causes significant changes in the microbiota. The carvacrol‐based product associated with C. jejuni inoculation increased the diversity and strongly modified the structure of the microbial community. Functional analysis by 16S rRNA gene‐based predictions further revealed that the product up‐regulated the pathways involved in the antimicrobial synthesis, which could explain its shaping effect on the caecal microbiota.
Conclusions
Our study confirmed the impairment of the caecal bacterial community after inoculation and demonstrated the ability of the product to reduce the C. jejuni load in chickens. Further investigations are needed to better understand the mode of action of this product to promote the installation of a beneficial microbiota to its host.
Significance and Impact of the Study
Results suggested that this product could be promising to control C. jejuni contamination of broilers.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>35278017</pmid><doi>10.1111/jam.15521</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0001-6366-2480</orcidid><orcidid>https://orcid.org/0000-0001-7000-3408</orcidid><orcidid>https://orcid.org/0000-0002-2945-4423</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of applied microbiology, 2022-06, Vol.132 (6), p.4501-4516 |
| issn | 1364-5072 1365-2672 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9314584 |
| source | Alma/SFX Local Collection |
| subjects | 16Sr RNA gene sequencing Animal biology Antiinfectives and antibacterials Bacteriology Biotechnology broiler chicken Campylobacter Campylobacter jejuni Carvacrol Chickens essential oil feed additive Food and Nutrition foodborne disease Functional analysis Gene sequencing Inoculation Life Sciences Microbiology and Parasitology microbiome Microbiota Microorganisms Mode of action Original PICRUSt2 Pollution control Poultry rRNA 16S Veterinary medicine and animal Health |
| title | A carvacrol‐based product reduces Campylobacter jejuni load and alters microbiota composition in the caeca of chickens |
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