Acute aerobic exercise‐conditioned serum reduces colon cancer cell proliferation in vitro through interleukin‐6‐induced regulation of DNA damage

Epidemiological evidence shows that regular physical activity is associated with reduced risk of primary and recurrent colon cancer. However, the underlying mechanisms of action are poorly understood. We evaluated the effects of stimulating a human colon cancer cell line (LoVo) with human serum coll...

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Bibliographic Details
Published in:International journal of cancer 2022-07, Vol.151 (2), p.265-274
Main Authors: Orange, Samuel T., Jordan, Alastair R., Odell, Adam, Kavanagh, Owen, Hicks, Kirsty M., Eaglen, Tristan, Todryk, Stephen, Saxton, John M.
Format: Article
Language:English
Subjects:
acute exercise
Aerobics
Body mass index
Cancer
cancer therapy
Cancer Therapy and Prevention
Cell growth
Cell proliferation
Colon cancer
Colorectal cancer
Deoxyribonucleic acid
DNA
DNA damage
DNA repair
Epidemiology
Exercise
exercise‐oncology
Heart rate
Interleukin 6
Medical research
Mimicry
Physical activity
Physical fitness
Physical training
Risk factors
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Summary:Epidemiological evidence shows that regular physical activity is associated with reduced risk of primary and recurrent colon cancer. However, the underlying mechanisms of action are poorly understood. We evaluated the effects of stimulating a human colon cancer cell line (LoVo) with human serum collected before and after an acute exercise bout vs nonexercise control serum on cancer cell proliferation. We also measured exercise‐induced changes in serum cytokines and intracellular protein expression to explore potential biological mechanisms. Blood samples were collected from 16 men with lifestyle risk factors for colon cancer (age ≥50 years; body mass index ≥25 kg/m2; physically inactive) before and immediately after an acute bout of moderate‐intensity aerobic interval exercise (6 × 5 minutes intervals at 60% heart rate reserve) and a nonexercise control condition. Stimulating LoVo cells with serum obtained immediately after exercise reduced cancer cell proliferation compared to control (−5.7%; P = .002). This was accompanied by a decrease in LoVo cell γ‐H2AX expression (−24.6%; P = .029), indicating a reduction in DNA damage. Acute exercise also increased serum IL‐6 (24.6%, P = .002). Furthermore, stimulating LoVo cells with recombinant IL‐6 reduced γ‐H2AX expression (β = −22.7%; P 
ISSN:0020-7136
1097-0215
DOI:10.1002/ijc.33982