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A pilot study on bacterial isolates associated with purulent vaginal discharge in dairy cows in the south‐west region of Western Australia
This study aimed to determine the bacterial isolates associated with postpartum endometritis among dairy cows in Western Australia and their antimicrobial susceptibility profiles. A cross‐sectional study was conducted between June–October 2020. Endometritis was defined as evidence of mucopurulent to...
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| Published in: | Australian veterinary journal 2022-05, Vol.100 (5), p.205-212 |
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| cites | cdi_FETCH-LOGICAL-c4432-63c696a09243be56cabd2bfd02044cd1e7f0f7ff4fa43863156268780ea101223 |
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| container_title | Australian veterinary journal |
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| creator | Ludbey, PA Sahibzada, S Annandale, CH Robertson, ID Waichigo, FK Tufail, MS Valenzuela, JL Aleri, JW |
| description | This study aimed to determine the bacterial isolates associated with postpartum endometritis among dairy cows in Western Australia and their antimicrobial susceptibility profiles. A cross‐sectional study was conducted between June–October 2020. Endometritis was defined as evidence of mucopurulent to purulent vaginal discharge 60–100 days postpartum. Vaginal discharge samples were obtained, cultured, identified and tested for antimicrobial susceptibility. A total of 118 bacterial isolates were grown from 46 animals, representing 36 species. The bacteria isolated from both aerobic and anaerobic cultures included Bacillus (60.2%), Streptococcus (12.7%), Trueperella (10.1%), Escherichia (6.7%) and Staphylococcus (5.9%). The remaining genera |
| doi_str_mv | 10.1111/avj.13152 |
| format | article |
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A cross‐sectional study was conducted between June–October 2020. Endometritis was defined as evidence of mucopurulent to purulent vaginal discharge 60–100 days postpartum. Vaginal discharge samples were obtained, cultured, identified and tested for antimicrobial susceptibility. A total of 118 bacterial isolates were grown from 46 animals, representing 36 species. The bacteria isolated from both aerobic and anaerobic cultures included Bacillus (60.2%), Streptococcus (12.7%), Trueperella (10.1%), Escherichia (6.7%) and Staphylococcus (5.9%). The remaining genera <5% were Histophilus, Aeroccocus, Enterococcus and Moraxella. Resistance was variable between isolates, but the highest resistance levels were observed in Streptococcal and Bacillus isolates to enrofloxacin, clindamycin and erythromycin, respectively. All Streptococcal isolates exhibited 100% resistance to enrofloxacin, and the greatest resistance levels were found in Streptococcus luteinises to trimethoprim‐sulfamethoxazole 83%, clindamycin 66% and 33% quinupristin‐dalfopristin. There was 84.5% resistance to clindamycin and 35.2% to erythromycin in the Bacillus isolates, with the highest resistance found in Bacillus licheniformis and Bacillus subtilis. Escherichia coli exhibited 12.5% resistance to gentamycin, ceftiofur, whereas amoxicillin‐clavulanic acid exhibited 37.5%. Within the Staphylococcal isolates, 28.5%, 28.5%, 42.8% and 14.2% resistance to ceftiofur, erythromycin, cefoxitin, penicillin and tetracycline were observed, respectively. The presence of resistance to important antimicrobials for human use, such as cephalosporins, macrolides and fluoroquinolones, highlights the need for judicious use of antimicrobials in dairy cattle.</description><identifier>ISSN: 0005-0423</identifier><identifier>EISSN: 1751-0813</identifier><identifier>DOI: 10.1111/avj.13152</identifier><identifier>PMID: 35243613</identifier><language>eng</language><publisher>Melbourne: Wiley Publishing Asia Pty Ltd</publisher><subject>Amoxicillin ; Animals ; Anti-Bacterial Agents - pharmacology ; Anti-Bacterial Agents - therapeutic use ; Antimicrobial agents ; antimicrobial resistance ; Bacillus ; Bacteria ; Cattle ; Cefoxitin ; Cephalosporins ; Clavulanic acid ; Clindamycin ; Cross-Sectional Studies ; Dairy cattle ; Dalfopristin ; Drug Resistance, Bacterial ; Endometritis ; Endometritis - drug therapy ; Endometritis - microbiology ; Endometritis - veterinary ; Enrofloxacin ; Erythromycin ; Escherichia coli ; Female ; Fluoroquinolones ; Gentamicin ; Humans ; Microbial Sensitivity Tests - veterinary ; Original ; Penicillin ; Pilot Projects ; Postpartum ; Production Animals ; Quinupristin ; Staphylococcus ; Streptococcus ; Sulfamethoxazole ; Trimethoprim ; Vagina ; Vaginal Discharge - drug therapy ; Vaginal Discharge - veterinary ; Western Australia - epidemiology</subject><ispartof>Australian veterinary journal, 2022-05, Vol.100 (5), p.205-212</ispartof><rights>2022 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Australian Veterinary Association.</rights><rights>2022 The Authors. Australian Veterinary Journal published by John Wiley & Sons Australia, Ltd on behalf of Australian Veterinary Association.</rights><rights>2022. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4432-63c696a09243be56cabd2bfd02044cd1e7f0f7ff4fa43863156268780ea101223</citedby><cites>FETCH-LOGICAL-c4432-63c696a09243be56cabd2bfd02044cd1e7f0f7ff4fa43863156268780ea101223</cites><orcidid>0000-0002-4498-2152</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35243613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ludbey, PA</creatorcontrib><creatorcontrib>Sahibzada, S</creatorcontrib><creatorcontrib>Annandale, CH</creatorcontrib><creatorcontrib>Robertson, ID</creatorcontrib><creatorcontrib>Waichigo, FK</creatorcontrib><creatorcontrib>Tufail, MS</creatorcontrib><creatorcontrib>Valenzuela, JL</creatorcontrib><creatorcontrib>Aleri, JW</creatorcontrib><title>A pilot study on bacterial isolates associated with purulent vaginal discharge in dairy cows in the south‐west region of Western Australia</title><title>Australian veterinary journal</title><addtitle>Aust Vet J</addtitle><description>This study aimed to determine the bacterial isolates associated with postpartum endometritis among dairy cows in Western Australia and their antimicrobial susceptibility profiles. A cross‐sectional study was conducted between June–October 2020. Endometritis was defined as evidence of mucopurulent to purulent vaginal discharge 60–100 days postpartum. Vaginal discharge samples were obtained, cultured, identified and tested for antimicrobial susceptibility. A total of 118 bacterial isolates were grown from 46 animals, representing 36 species. The bacteria isolated from both aerobic and anaerobic cultures included Bacillus (60.2%), Streptococcus (12.7%), Trueperella (10.1%), Escherichia (6.7%) and Staphylococcus (5.9%). The remaining genera <5% were Histophilus, Aeroccocus, Enterococcus and Moraxella. Resistance was variable between isolates, but the highest resistance levels were observed in Streptococcal and Bacillus isolates to enrofloxacin, clindamycin and erythromycin, respectively. All Streptococcal isolates exhibited 100% resistance to enrofloxacin, and the greatest resistance levels were found in Streptococcus luteinises to trimethoprim‐sulfamethoxazole 83%, clindamycin 66% and 33% quinupristin‐dalfopristin. There was 84.5% resistance to clindamycin and 35.2% to erythromycin in the Bacillus isolates, with the highest resistance found in Bacillus licheniformis and Bacillus subtilis. Escherichia coli exhibited 12.5% resistance to gentamycin, ceftiofur, whereas amoxicillin‐clavulanic acid exhibited 37.5%. Within the Staphylococcal isolates, 28.5%, 28.5%, 42.8% and 14.2% resistance to ceftiofur, erythromycin, cefoxitin, penicillin and tetracycline were observed, respectively. The presence of resistance to important antimicrobials for human use, such as cephalosporins, macrolides and fluoroquinolones, highlights the need for judicious use of antimicrobials in dairy cattle.</description><subject>Amoxicillin</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Antimicrobial agents</subject><subject>antimicrobial resistance</subject><subject>Bacillus</subject><subject>Bacteria</subject><subject>Cattle</subject><subject>Cefoxitin</subject><subject>Cephalosporins</subject><subject>Clavulanic acid</subject><subject>Clindamycin</subject><subject>Cross-Sectional Studies</subject><subject>Dairy cattle</subject><subject>Dalfopristin</subject><subject>Drug Resistance, Bacterial</subject><subject>Endometritis</subject><subject>Endometritis - drug therapy</subject><subject>Endometritis - microbiology</subject><subject>Endometritis - veterinary</subject><subject>Enrofloxacin</subject><subject>Erythromycin</subject><subject>Escherichia coli</subject><subject>Female</subject><subject>Fluoroquinolones</subject><subject>Gentamicin</subject><subject>Humans</subject><subject>Microbial Sensitivity Tests - veterinary</subject><subject>Original</subject><subject>Penicillin</subject><subject>Pilot Projects</subject><subject>Postpartum</subject><subject>Production Animals</subject><subject>Quinupristin</subject><subject>Staphylococcus</subject><subject>Streptococcus</subject><subject>Sulfamethoxazole</subject><subject>Trimethoprim</subject><subject>Vagina</subject><subject>Vaginal Discharge - drug therapy</subject><subject>Vaginal Discharge - veterinary</subject><subject>Western Australia - epidemiology</subject><issn>0005-0423</issn><issn>1751-0813</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNp1kcuOEzEQRS0EYsLAgh9AltjAomfKz-5skKIRT43EhsfScrvdiaNOO9juRNnxASz4Rr6EChlGgIQ3dslHt6ruJeQxgwuG59Lu1hdMMMXvkBmrFaugYeIumQGAqkBycUYe5LwGELXi6j45E4pLoZmYkW8Lug1DLDSXqTvQONLWuuJTsAMNOQ62-ExtztEFfHZ0H8qKbqc0DX4sdGeXYUSyC9mtbFp6Gkba2ZAO1MV9PlZl5WmOU1n9-Pp973OhyS8Dtok9_YylTyNdTLkkOwT7kNzr7ZD9o5v7nHx89fLD1Zvq-v3rt1eL68pJKXilhdNzbWGOS7ReaWfbjrd9BxykdB3zdQ993feyt1I0Go3RXDd1A94yYJyLc_LipLud2o3vHK6C_c02hY1NBxNtMH__jGFllnFn5qgFIFHg2Y1Ail8mXMNs0AE_DHb0ccqGa3RX4nyA6NN_0HWcEpp2pFQDSjV6jtTzE-VSzDn5_nYYBuaYscGMza-MkX3y5_S35O9QEbg8Afsw-MP_lczi07uT5E9WoLP6</recordid><startdate>202205</startdate><enddate>202205</enddate><creator>Ludbey, PA</creator><creator>Sahibzada, S</creator><creator>Annandale, CH</creator><creator>Robertson, ID</creator><creator>Waichigo, FK</creator><creator>Tufail, MS</creator><creator>Valenzuela, JL</creator><creator>Aleri, JW</creator><general>Wiley Publishing Asia Pty Ltd</general><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4498-2152</orcidid></search><sort><creationdate>202205</creationdate><title>A pilot study on bacterial isolates associated with purulent vaginal discharge in dairy cows in the south‐west region of Western Australia</title><author>Ludbey, PA ; Sahibzada, S ; Annandale, CH ; Robertson, ID ; Waichigo, FK ; Tufail, MS ; Valenzuela, JL ; Aleri, JW</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4432-63c696a09243be56cabd2bfd02044cd1e7f0f7ff4fa43863156268780ea101223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amoxicillin</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Anti-Bacterial Agents - therapeutic use</topic><topic>Antimicrobial agents</topic><topic>antimicrobial resistance</topic><topic>Bacillus</topic><topic>Bacteria</topic><topic>Cattle</topic><topic>Cefoxitin</topic><topic>Cephalosporins</topic><topic>Clavulanic acid</topic><topic>Clindamycin</topic><topic>Cross-Sectional Studies</topic><topic>Dairy cattle</topic><topic>Dalfopristin</topic><topic>Drug Resistance, Bacterial</topic><topic>Endometritis</topic><topic>Endometritis - drug therapy</topic><topic>Endometritis - microbiology</topic><topic>Endometritis - veterinary</topic><topic>Enrofloxacin</topic><topic>Erythromycin</topic><topic>Escherichia coli</topic><topic>Female</topic><topic>Fluoroquinolones</topic><topic>Gentamicin</topic><topic>Humans</topic><topic>Microbial Sensitivity Tests - veterinary</topic><topic>Original</topic><topic>Penicillin</topic><topic>Pilot Projects</topic><topic>Postpartum</topic><topic>Production Animals</topic><topic>Quinupristin</topic><topic>Staphylococcus</topic><topic>Streptococcus</topic><topic>Sulfamethoxazole</topic><topic>Trimethoprim</topic><topic>Vagina</topic><topic>Vaginal Discharge - drug therapy</topic><topic>Vaginal Discharge - veterinary</topic><topic>Western Australia - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ludbey, PA</creatorcontrib><creatorcontrib>Sahibzada, S</creatorcontrib><creatorcontrib>Annandale, CH</creatorcontrib><creatorcontrib>Robertson, ID</creatorcontrib><creatorcontrib>Waichigo, FK</creatorcontrib><creatorcontrib>Tufail, MS</creatorcontrib><creatorcontrib>Valenzuela, JL</creatorcontrib><creatorcontrib>Aleri, JW</creatorcontrib><collection>Wiley-Blackwell Open Access Collection</collection><collection>Wiley Online Library Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Australian veterinary journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ludbey, PA</au><au>Sahibzada, S</au><au>Annandale, CH</au><au>Robertson, ID</au><au>Waichigo, FK</au><au>Tufail, MS</au><au>Valenzuela, JL</au><au>Aleri, JW</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A pilot study on bacterial isolates associated with purulent vaginal discharge in dairy cows in the south‐west region of Western Australia</atitle><jtitle>Australian veterinary journal</jtitle><addtitle>Aust Vet J</addtitle><date>2022-05</date><risdate>2022</risdate><volume>100</volume><issue>5</issue><spage>205</spage><epage>212</epage><pages>205-212</pages><issn>0005-0423</issn><eissn>1751-0813</eissn><abstract>This study aimed to determine the bacterial isolates associated with postpartum endometritis among dairy cows in Western Australia and their antimicrobial susceptibility profiles. A cross‐sectional study was conducted between June–October 2020. Endometritis was defined as evidence of mucopurulent to purulent vaginal discharge 60–100 days postpartum. Vaginal discharge samples were obtained, cultured, identified and tested for antimicrobial susceptibility. A total of 118 bacterial isolates were grown from 46 animals, representing 36 species. The bacteria isolated from both aerobic and anaerobic cultures included Bacillus (60.2%), Streptococcus (12.7%), Trueperella (10.1%), Escherichia (6.7%) and Staphylococcus (5.9%). The remaining genera <5% were Histophilus, Aeroccocus, Enterococcus and Moraxella. Resistance was variable between isolates, but the highest resistance levels were observed in Streptococcal and Bacillus isolates to enrofloxacin, clindamycin and erythromycin, respectively. All Streptococcal isolates exhibited 100% resistance to enrofloxacin, and the greatest resistance levels were found in Streptococcus luteinises to trimethoprim‐sulfamethoxazole 83%, clindamycin 66% and 33% quinupristin‐dalfopristin. There was 84.5% resistance to clindamycin and 35.2% to erythromycin in the Bacillus isolates, with the highest resistance found in Bacillus licheniformis and Bacillus subtilis. Escherichia coli exhibited 12.5% resistance to gentamycin, ceftiofur, whereas amoxicillin‐clavulanic acid exhibited 37.5%. Within the Staphylococcal isolates, 28.5%, 28.5%, 42.8% and 14.2% resistance to ceftiofur, erythromycin, cefoxitin, penicillin and tetracycline were observed, respectively. The presence of resistance to important antimicrobials for human use, such as cephalosporins, macrolides and fluoroquinolones, highlights the need for judicious use of antimicrobials in dairy cattle.</abstract><cop>Melbourne</cop><pub>Wiley Publishing Asia Pty Ltd</pub><pmid>35243613</pmid><doi>10.1111/avj.13152</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-4498-2152</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Amoxicillin Animals Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Antimicrobial agents antimicrobial resistance Bacillus Bacteria Cattle Cefoxitin Cephalosporins Clavulanic acid Clindamycin Cross-Sectional Studies Dairy cattle Dalfopristin Drug Resistance, Bacterial Endometritis Endometritis - drug therapy Endometritis - microbiology Endometritis - veterinary Enrofloxacin Erythromycin Escherichia coli Female Fluoroquinolones Gentamicin Humans Microbial Sensitivity Tests - veterinary Original Penicillin Pilot Projects Postpartum Production Animals Quinupristin Staphylococcus Streptococcus Sulfamethoxazole Trimethoprim Vagina Vaginal Discharge - drug therapy Vaginal Discharge - veterinary Western Australia - epidemiology |
| title | A pilot study on bacterial isolates associated with purulent vaginal discharge in dairy cows in the south‐west region of Western Australia |
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