Microscale Thermophoresis as a Tool to Study Protein Interactions and Their Implication in Human Diseases

The review highlights how protein–protein interactions (PPIs) have determining roles in most life processes and how interactions between protein partners are involved in various human diseases. The study of PPIs and binding interactions as well as their understanding, quantification and pharmacologi...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-07, Vol.23 (14), p.7672
Main Authors: Magnez, Romain, Bailly, Christian, Thuru, Xavier
Format: Article
Language:English
Subjects:
Antibodies
Binding
Binding sites
Bioavailability
Biochemistry, Molecular Biology
Bioengineering
Cancer
Case studies
Computer applications
Enzymes
High-throughput screening
Human health and pathology
Infectious diseases
Life Sciences
Ligands
Peptides
Protein interaction
Proteins
Review
Signal transduction
Therapeutic applications
Thermophoresis
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Summary:The review highlights how protein–protein interactions (PPIs) have determining roles in most life processes and how interactions between protein partners are involved in various human diseases. The study of PPIs and binding interactions as well as their understanding, quantification and pharmacological regulation are crucial for therapeutic purposes. Diverse computational and analytical methods, combined with high-throughput screening (HTS), have been extensively used to characterize multiple types of PPIs, but these procedures are generally laborious, long and expensive. Rapid, robust and efficient alternative methods are proposed, including the use of Microscale Thermophoresis (MST), which has emerged as the technology of choice in drug discovery programs in recent years. This review summarizes selected case studies pertaining to the use of MST to detect therapeutically pertinent proteins and highlights the biological importance of binding interactions, implicated in various human diseases. The benefits and limitations of MST to study PPIs and to identify regulators are discussed.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23147672