Diversity and distribution in hypersaline microbial mats of bacteria related to Chloroflexus spp

Filamentous bacteria containing bacteriochlorophylls c and a were enriched from hypersaline microbial mats. Based on phylogenetic analyses of 16S rRNA gene sequences, these organisms form a previously undescribed lineage distantly related to Chloroflexus spp. We developed and tested a set of PCR pri...

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Bibliographic Details
Published in:Applied and environmental microbiology 2001-09, Vol.67 (9), p.4365-4371
Main Authors: NÜBEL, Ulrich, BATESON, Mary M, MADIGAN, Michael T, KÜHL, Michael, WARD, David M
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Bacteria
Biodiversity
Biological and medical sciences
Brackish
Chlorobi - classification
Chlorobi - genetics
Chlorobi - growth & development
Chloroflexus
Chlorophyll
Culture Media
DNA Primers
Ecosystem
Fundamental and applied biological sciences. Psychology
Genes
Genes, rRNA
Habitats
Marine
Microbial Ecology
Microbiology
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sequence Analysis, DNA
Sodium Chloride
Various environments (extraatmospheric space, air, water)
Water Microbiology
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Summary:Filamentous bacteria containing bacteriochlorophylls c and a were enriched from hypersaline microbial mats. Based on phylogenetic analyses of 16S rRNA gene sequences, these organisms form a previously undescribed lineage distantly related to Chloroflexus spp. We developed and tested a set of PCR primers for the specific amplification of 16S rRNA genes from filamentous phototrophic bacteria within the kingdom of "green nonsulfur bacteria." PCR products recovered from microbial mats in a saltern in Guerrero Negro, Mexico, were subjected to cloning or denaturing gradient gel electrophoresis and then sequenced. We found evidence of a high diversity of bacteria related to Chloroflexus which exhibit different distributions along a gradient of salinity from 5.5 to 16%.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.67.9.4365-4371.2001