A Panel of Eight miRNAs Is Deregulated in HTLV-2 Infected PBMCs and BJABGu Cell Line

Despite human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 being retroviruses closely related at a genomic level, HTLV-2 differs from HTLV-1 in terms of pathogenicity in both single infection and coinfection contexts. Moreover, the HTLV-2 association with clinical outcomes is still debated and s...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-07, Vol.23 (14), p.7583
Main Authors: Pilotti, Elisabetta, Cannata, Attilio, Magnani, Giacomo, Bignami, Fabio, Corsi, Andrea, Valenti, Maria Teresa, Shallak, Mariam, Forlani, Greta, Romanelli, Maria Grazia
Format: Article
Language:English
Subjects:
Cbfa-1 protein
Cell differentiation
Cytokines
Deregulation
Differentiation (biology)
Gene expression
Gene regulation
Immune response
Infections
Leukemia
Lymphocytes T
Microenvironments
miRNA
Pathogenicity
Peripheral blood mononuclear cells
Post-transcription
Proteins
Signal transduction
Transcription factors
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Summary:Despite human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 being retroviruses closely related at a genomic level, HTLV-2 differs from HTLV-1 in terms of pathogenicity in both single infection and coinfection contexts. Moreover, the HTLV-2 association with clinical outcomes is still debated and several mechanisms underlying HTLV-2 infection remain unexplored as well. Cellular miRNAs are key factors in the post-transcriptional regulation of gene expression and they are known to be potential targets for several pathogens to control the host microenvironment and, in particular, escape immune responses. Here, we identified a HTLV-2-related signature of eight miRNAs (miR-125a-3p, miR-381-3p, miR-502-5p, miR-708-5p, miR-548d-5p, miR-548c-5p, miR-1-3p, and miR-511-5p) in both HTLV-2 infected PBMC and BJABGu cell lines. Altered miRNA expression patterns were correlated with the impairment of Th cell differentiation and signaling pathways driven by cytokines and transcriptional factors such as the Runt-related transcription factor (RUNX) family members. Specifically, we demonstrated that the RUNX2 protein was significantly more expressed in the presence of Tax-2 compared with Tax-1 in an in vitro cell model. To the best of our knowledge, these data represent the first contribution to elucidating the HTLV-2 mediated alteration of host cell miRNA profiles that may impact on HTLV-2 replication and persistent infection.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23147583