New additive for culture media for rapid identification of aflatoxin-producing Aspergillus strains

A new reliable, fast, and simple method for the detection of aflatoxigenic Aspergillus strains, consisting of the addition of a cyclodextrin (a methylated beta-cyclodextrin derivative) to common media used for testing mycotoxin production ability, was developed. We propose the use of this compound a...

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Bibliographic Details
Published in:Applied and environmental microbiology 2001-10, Vol.67 (10), p.4858-4862
Main Authors: FENTE, C. A, JAIMEZ ORDAZ, J, VAZQUEZ, B. I, FRANCO, C. M, CEPEDA, A
Format: Article
Language:English
Subjects:
Additives
Aflatoxins - biosynthesis
Aspergillus
Aspergillus - classification
Aspergillus - growth & development
Aspergillus - metabolism
Biological and medical sciences
Culture Media - chemistry
cyclodextrins
Cyclodextrins - metabolism
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
Fungi
Methods
Mycological Typing Techniques
Poisons
Test methods
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Summary:A new reliable, fast, and simple method for the detection of aflatoxigenic Aspergillus strains, consisting of the addition of a cyclodextrin (a methylated beta-cyclodextrin derivative) to common media used for testing mycotoxin production ability, was developed. We propose the use of this compound as an additive for fungal culture media to enhance the natural fluorescence of aflatoxins. The production of aflatoxins coincided with the presence of a bright blue or blue-green fluorescent area surrounding colonies when observed under long-wavelength (365-nm) UV light after 3 days of incubation at 28 degrees C. The presence of aflatoxins was confirmed by extracting the medium with chloroform and examining the extracts by high-pressure liquid chromatography with fluorescence detection.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.67.10.4858-4862.2001