Variation in resistance to high hydrostatic pressure and rpoS heterogeneity in natural isolates of Escherichia coli O157:H7

Several natural isolates of Escherichia coli O157:H7 have previously been shown to exhibit stationary-phase-dependent variation in their resistance to inactivation by high hydrostatic pressure. In this report we demonstrate that loss of the stationary-phase-inducible sigma factor RpoS resulted in de...

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Bibliographic Details
Published in:Applied and environmental microbiology 2001-10, Vol.67 (10), p.4901-4907
Main Authors: ROBEY, Marianne, BENITO, Amparo, HUTSON, Roger H, PASCUAL, Cristina, PARK, Simon F, MACKEY, Bernard M
Format: Article
Language:English
Subjects:
Alleles
Animals
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Base Sequence
Biological and medical sciences
Cattle
Cloning, Molecular
Escherichia coli
Escherichia coli O157 - genetics
Escherichia coli O157 - growth & development
Escherichia coli O157 - isolation & purification
Food industries
Food Microbiology
Fundamental and applied biological sciences. Psychology
Genetic Variation
Humans
Hydrostatic Pressure
Molecular Sequence Data
rpoS gene
Sequence Analysis, DNA
Sigma Factor - genetics
Sigma Factor - metabolism
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Summary:Several natural isolates of Escherichia coli O157:H7 have previously been shown to exhibit stationary-phase-dependent variation in their resistance to inactivation by high hydrostatic pressure. In this report we demonstrate that loss of the stationary-phase-inducible sigma factor RpoS resulted in decreased resistance to pressure in E. coli O157:H7 and in a commensal strain. Furthermore, variation in the RpoS activity of the natural isolates of O157:H7 correlated with the pressure resistance of those strains. Heterogeneity was noted in the rpoS alleles of the natural isolates that may explain the differences in RpoS activity. These results are consistent with a role for rpoS in mediating resistance to high hydrostatic pressure in E. coli O157:H7.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.67.10.4901-4907.2001