Synovial fluid niche promoted differentiation of dental follicle mesenchymal stem cells toward chondrogenesis in rheumatoid arthritis

Objectives: In this study, we aimed to investigate the differentiation potential of dental follicle mesenchymal stem cells (MSCs) in the synovial fluid (SF) niche of early-onset or end-stage rheumatoid arthritis (RA). Patients and methods: Between May 2020 and January 2021, six patients (1 male, 5 f...

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Bibliographic Details
Published in:Archives of rheumatology 2022-03, Vol.37 (1), p.94-109
Main Authors: Genc, Deniz, Kurkcu, Merve Sezer, Yigitturk, Gurkan, Gunaydin, Burcu, Elbe, Hulya, Aladag, Akin, Col, Bekir, Tarhan, Emine Figen
Format: Article
Language:English
Subjects:
Analysis
Arthritis
Biological response modifiers
Collagen
Gene expression
Health aspects
Instrument industry
Interferon
Interleukins
Original
Rheumatoid factor
RNA
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Summary:Objectives: In this study, we aimed to investigate the differentiation potential of dental follicle mesenchymal stem cells (MSCs) in the synovial fluid (SF) niche of early-onset or end-stage rheumatoid arthritis (RA). Patients and methods: Between May 2020 and January 2021, six patients (1 male, 5 females; mean age: 57.5 [+ or -] 11.2 years; range, 49 to 65 years) who were diagnosed with RA with the indication of SF aspiration were included in the study. The third passage dental follicle stem cells (DFSCs) were cocultured with fresh SF samples of end-stage or early-onset RA patients in micromass culture system for 21 days. SF samples were analyzed for secreted cytokines. Chondrogenic markers (CD49e, CD49f) were analyzed in DFSCs, gene expression analysis was performed for the expressions of Col I, Col II, Aggrecan and Sox-9, and histochemical analysis was performed by staining three-dimensional pellets with anti-collagen II antibody. The neutralization assay was performed with anti-interleukin (IL)-6, anti-interferon-gamma (IFN-[gamma]), and anti-IL-1beta([beta]). Results: The high levels of IL-1[beta] and IL-6 were observed in end-stage RA patients' SF samples compared to the early-onset patients (p
ISSN:2148-5046
2618-6500
DOI:10.46497/ArchRheumatol.2022.8891