Recovery and phylogenetic analysis of nifH sequences from diazotrophic bacteria associated with dead aboveground biomass of Spartina alterniflora

DNA was extracted from dry standing dead Spartina alterniflora stalks as well as dry Spartina wrack from the North Inlet (South Carolina) and Sapelo Island (Georgia) salt marshes. Partial nifH sequences were PCR amplified, the products were separated by denaturing gradient gel electrophoresis (DGGE)...

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Bibliographic Details
Published in:Applied and environmental microbiology 2001-11, Vol.67 (11), p.5308-5314
Main Authors: LOVELL, Charles R, FRIEZ, Michael J, LONGSHORE, John W, BAGWELL, Christopher E
Format: Article
Language:English
Subjects:
Alphaproteobacteria - genetics
Animal, plant and microbial ecology
Azospirillum brasilense
Bacteria
Biological and medical sciences
Biomass
Brackish
Decomposition
DNA, Bacterial - analysis
DNA, Bacterial - isolation & purification
Electrophoresis, Polyacrylamide Gel - methods
Fundamental and applied biological sciences. Psychology
Gammaproteobacteria - genetics
Grasses
Marine
Microbial Ecology
Microbiology
Microflora of plants
Molecular Sequence Data
Oxidoreductases - genetics
Phylogeny
Poaceae - microbiology
Poaceae - physiology
Polymerase Chain Reaction - methods
Proteobacteria
Sequence Analysis, DNA
Spartina alterniflora
Wetlands
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Summary:DNA was extracted from dry standing dead Spartina alterniflora stalks as well as dry Spartina wrack from the North Inlet (South Carolina) and Sapelo Island (Georgia) salt marshes. Partial nifH sequences were PCR amplified, the products were separated by denaturing gradient gel electrophoresis (DGGE), and the prominent DGGE bands were sequenced. Most sequences (109 of 121) clustered with those from alpha-Proteobacteria, and 4 were very similar (>99%) to that of Azospirillum brasilense. Seven sequences clustered with those from known gamma-Proteobacteria and five with those from known anaerobic diazotrophs. The diazotroph assemblages associated with dead Spartina biomass in these two salt marshes were very similar, and relatively few major lineages were represented.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.67.11.5308-5314.2001