Bovine serum albumin adsorbed on eremomycin and grafted on silica as new mixed-binary chiral sorbent for improved enantioseparation of drugs

A new silica-based, mixed-binary chiral sorbent grafted with the macrocyclic antibiotic eremomycin and bovine serum albumin (BSA) was obtained. The sorbent-filled high-performance liquid chromatography column was capable of enantioseparation of racemic drugs, such as profens, in reversed-phase-chrom...

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Published in:Yàowu shi͡p︡in fenxi 2016-10, Vol.24 (4), p.848-854
Main Authors: Fedorova, Irina A., Shapovalova, Elena N., Shpigun, Oleg A., Staroverov, Sergey M.
Format: Article
Language:English
Subjects:
Amino acids
Antibiotics
binary chiral sorbent
Bovine serum albumin
chiral stationary phase
Chromatography
Drugs
eremomycin
Glycopeptides
Grafting
High performance liquid chromatography
Liquid chromatography
Nonsteroidal anti-inflammatory drugs
Original
Pharmaceutical industry
Physiology
profen
Proteins
Serum albumin
Serum Albumin, Bovine - chemistry
Silica
Silicon Dioxide
Sodium
Stereoisomerism
Urine
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Summary:A new silica-based, mixed-binary chiral sorbent grafted with the macrocyclic antibiotic eremomycin and bovine serum albumin (BSA) was obtained. The sorbent-filled high-performance liquid chromatography column was capable of enantioseparation of racemic drugs, such as profens, in reversed-phase-chromatography mode. The mixed-binary eremomycin-BSA-sorbent showed better capability for profens enantioseparation as compared with a sorbent containing eremomycin only. BSA grafted onto the sorbent surface significantly reduced retention times of other proteins from the analyte solution, and free proteins (including BSA) injected as analytes were not retained on the column, and subsequently eluted with a dead volume. The drastic difference observed in the binding of profens and other proteins using the sorbent was tested for determination and enantioseparation of profens in artificial-urine solutions. [Display omitted]
ISSN:1021-9498
2224-6614
DOI:10.1016/j.jfda.2016.03.011