Anti-inflammatory effects, nuclear magnetic resonance identification, and high-performance liquid chromatography isolation of the total flavonoids from Artemisia frigida

• Published in: Yàowu shi͡p︡in fenxi 2016-04, Vol.24 (2), p.385-391
• Main Authors: Wang, Qinghu, Jin, Jinmei, Dai, Nayintai, Han, Narenchaoketu, Han, Jingjing, Bao, Baiyinmuqier
• Format: Article
• Language: English
• Subjects: Animals; anti-inflammatory; Anti-Inflammatory Agents; Antiinflammatory agents; Artemisia; Artemisia frigida; Arthritis; Bioactive compounds; Carrageenan; Chromatography; Chromatography, High Pressure Liquid; Drug dosages; Edema; Ethanol; Flavonoids; High performance liquid chromatography; high-performance liquid chromatography analysis; Inflammation; Laboratory animals; Liquid chromatography; Magnetic Resonance Spectroscopy; Menstruation; Narcotics; NMR; Nonsteroidal anti-inflammatory drugs; Nuclear magnetic resonance; Original; Plant Extracts; Rats
• ISSN: 1021-9498; 2224-6614
• DOI: 10.1016/j.jfda.2015.11.004

The aerial parts of Artemisia frigida Willd. are used to treat joint swelling, renal heat, abnormal menstruation, and sore carbuncle. The anti-inflammatory effects of A. frigida have been well-known in folk medicine, suggesting that components extracted from A. frigida could potentially treat inflammatory disease. With the aim of discovering bioactive compounds, in this study, we extracted total flavonoids from the aerial parts of A. frigida and investigated their anti-inflammatory effects against inflammation induced by carrageenan and egg albumin in rats. At the doses studied, total flavonoids (100 mg/kg, 200 mg/kg, and 400 mg/kg) and some isolated compounds (30 mg/kg) showed significant and dose-dependent anti-inflammatory effects. According to the high-performance liquid chromatography analysis of the total flavonoids from A. frigida, there are five major compounds, namely, 5-hydroxy-3′,4′-dimethoxy-7-O-β-d-glucuronide (F1), 5-hydroxy-3′,4′,5′-trimethoxy-7-O-β-d-glucuronide (F2), 5,7,3′-trihydroxy-6,4′-dimethoxyflavone (F3), 5,3′-dihydroxy-6,7,4′-trimethoxyflavone (F4), and 5,3′-dihydroxy-3,6,7,4′-tetramethoxyflavone (F5), which may explain the anti-inflammatory activity. [Display omitted]