Towards Control and Oversight of SARS‐CoV‐2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors

The development of versatile and sensitive biotools to quantify specific SARS‐CoV‐2 immunoglobulins in SARS‐CoV‐2 infected and non‐infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in‐house expressed recombinant spike (S) proteins is reported....

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2022-07, Vol.61 (28), p.e202203662-n/a
Main Authors: Torrente‐Rodríguez, Rebeca M., Montero‐Calle, Ana, San Bartolomé, Clara, Cano, Olga, Vázquez, Mónica, Iglesias‐Caballero, María, Corral‐Lugo, Andrés, McConnell, Michael J., Pascal, Mariona, Mas, Vicente, Pingarrón, José M., Barderas, Rodrigo, Campuzano, Susana
Format: Article
Language:English
Subjects:
Antibodies
Biosensors
Electrical measurement
Electroanalytical Bioplatforms
Hydrogen peroxide
Immune Response
Immune system
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Immunoglobulins
Infections
Interrogation
Isotypes
Microspheres
Monitoring
Multiplexing
Nanoparticles
Nucleocapsids
Omicron
SARS-Cov-2
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Vaccines
Viral Antigens
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Summary:The development of versatile and sensitive biotools to quantify specific SARS‐CoV‐2 immunoglobulins in SARS‐CoV‐2 infected and non‐infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in‐house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N‐ and S‐specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP‐conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen‐printed electrodes using the HQ/HRP/H2O2 system. The obtained results using N and in‐house expressed S ectodomains of five SARS‐CoV‐2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency, and even identification of the variant responsible for the infection. A multiplexed amperometric bioplatform can detect and quantify total and isotype N‐ and S‐specific anti‐SARS‐CoV‐2 serum immunoglobulins within 75 minutes. The platform was used to analyze the global and isotype‐specific immune response of COVID‐19 convalescent and vaccinated individuals, and to detect variant‐specific S antibodies from variants of concern.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202203662