Immunophenotyping of lymphocytes and monocytes and the status of cytokines in the clinical course of Covid‐19 patients

Lymphopenia, T cell subgroup changes, and cytokine level differences are important in the early diagnosis and treatment of Covid‐19 cases and similar pandemics. We aimed to investigate the T cell, monocyte subgroups, and cytokine differences according to disease severity. A total of 46 volunteers we...

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Bibliographic Details
Published in:Journal of medical virology 2022-10, Vol.94 (10), p.4744-4753
Main Authors: Idiz, Ufuk O., Yurttas, Tahir T., Degirmencioglu, Serife, Orhan, Bagnu, Erdogan, Emre, Sevik, Husnu, Sevinc, Mert M.
Format: Article
Language:English
Subjects:
Blood
CD3 antigen
CD4 antigen
CD8 antigen
cell‐mediated immunity
Correlation
COVID-19
Cytokines
Flow cytometry
Heart rate
Interleukin 6
Leukocytes
Leukocytes (neutrophilic)
lymphocyte
Lymphocytes
Lymphocytes T
Lymphopenia
monocyte
Monocyte chemoattractant protein
Monocytes
Natural killer cells
Neutrophils
Oxygen content
Pandemics
Proteins
Subgroups
Tumor necrosis factor
Virology
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Summary:Lymphopenia, T cell subgroup changes, and cytokine level differences are important in the early diagnosis and treatment of Covid‐19 cases and similar pandemics. We aimed to investigate the T cell, monocyte subgroups, and cytokine differences according to disease severity. A total of 46 volunteers were included in the study. According to disease status, there were three groups (control, mild, and severe). The age, gender, smoking status, temperature, heart rate and oxygen saturation, complete blood count, C‐reactive protein (CRP) was noted, and flow cytometric analyses were performed for T cell and monocyte subgroups, and cytokine levels. Temperature, heart rate, SPO2, white blood cell (WBC), lympocyte count, trombocyte count, neutrophil/lymphocyte ratio, D‐dimer and CRP levels, lymphocyte %, lymphocyte/monocyte rate, monocyte subtypes (%), CD3+, CD4+, CD8+ cell counts, interleukin (IL)‐1β, TNF‐alpha, monocyte chemoattractant protein (MCP)‐1, IL‐6, IL‐8, IL‐10, IL‐18, IL‐23 were significantly different between groups. CRP, IL‐8, neutrophil/lymphocyte ratio, NK cells (%) have positive correlation and negative correlation was observed at lymphocyte (count), lymphocyte (%), lymphocyte/monocyte, classical monocyte (%), lymphocyte (count), CD3+ (count), CD4+ (count). As conclusion, lymphocyte (%), Lymphocyte (count), CRP levels, CD3+ and CD4+ cell counts strongly correlate with disease severity are valuable parameters for determining the prognoses of Covid‐19.
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.27917