Effects of aqueous extract of Ruta graveolens and its ingredients on cytochrome P450, uridine diphosphate (UDP)-glucuronosyltransferase, and reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H)-quinone oxidoreductase in mice

• Published in: Yàowu shi͡p︡in fenxi 2015-09, Vol.23 (3), p.516-528
• Main Authors: Ueng, Yune-Fang, Chen, Chien-Chih, Huang, Yu-Ling, Lee, I-Jung, Yun, Chul-Ho, Chen, Yu-Hsuan, Huang, Chiung-Chiao
• Format: Article
• Language: English
• Subjects: Chromatography; CYP1A protein; Cytochrome; Cytochrome P450; Drug dosages; Enzymes; Females; Furanocoumarins; Ingredients; Males; Metabolism; Mice; Motility; NAD(P)H:quinone oxidoreductase; Nicotinamide; Nicotinamide adenine dinucleotide; Oral administration; Original; Phosphates; Physiology; Polyclonal antibodies; Proteins; Quinone oxidoreductase; Rodents; Ruta graveolens; Rutin; Sperm; Therapeutic applications; UDP-glucuronosyltransferase; Uridine
• ISSN: 1021-9498; 2224-6614
• DOI: 10.1016/j.jfda.2015.03.005

Ruta graveolens (the common rue) has been used for various therapeutic purposes, including relief of rheumatism and treatment of circulatory disorder. To elucidate the effects of rue on main drug-metabolizing enzymes, effects of an aqueous extract of the aerial part of rue and its ingredients on cytochrome P450 (P450/CYP), uridine diphosphate (UDP)-glucuronosyltransferase, and reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H):quinone oxidoreductase were studied in C57BL/6JNarl mice. Oral administration of rue extract to males increased hepatic Cyp1a and Cyp2b activities in a dose-dependent manner. Under a 7-day treatment regimen, rue extract (0.5 g/kg) induced hepatic Cyp1a and Cyp2b activities and protein levels in males and females. This treatment increased hepatic UDP-glucuronosyltransferase activity only in males. However, NAD(P)H:quinone oxidoreductase activity remained unchanged. Based on the contents of rutin and furanocoumarins of mouse dose of rue extract, rutin increased hepatic Cyp1a activity and the mixture of furanocoumarins (Fmix) increased Cyp2b activities in males. The mixture of rutin and Fmix increased Cyp1a and Cyp2b activities. These results revealed that rutin and Fmix contributed at least in part to the P450 induction by rue.