Characterization of the divergent sacBK and sacAR operons, involved in sucrose utilization by Lactococcus lactis

The divergently transcribed sacBK and sacAR operons, which are involved in the utilization of sucrose by Lactococcus lactis NZ9800, were examined by transcriptional and gene inactivation studies. Northern analyses of RNA isolated from cells grown at the expense of different carbon sources revealed t...

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Bibliographic Details
Published in:Journal of bacteriology 1999-03, Vol.181 (6), p.1924-1926
Main Authors: Luesink, E.J, Marugg, J.D, Kuipers, O.P, Vos, W.M. de
Format: Article
Language:English
Subjects:
Bacteria
Bacteriology
beta-Fructofuranosidase
carbohydrate metabolism
fructokinase
Fructokinases - genetics
Fructokinases - metabolism
genbank/z97015
gene expression
Genes
Genes, Bacterial
genetic regulation
Genetics and Molecular Biology
glucose
Glycoside Hydrolases - genetics
Glycoside Hydrolases - metabolism
Hexosyltransferases - genetics
Hexosyltransferases - metabolism
kinases
Lactococcus lactis
Lactococcus lactis - genetics
Lactococcus lactis - metabolism
messenger RNA
Molecular Sequence Data
Multigene Family
nucleotide sequences
Operon
regulator genes
Repressor Proteins - genetics
Repressor Proteins - metabolism
Ribonucleic acid
RNA
structural genes
sucrose
Sucrose - metabolism
transcription (genetics)
transcription factors
Transcription, Genetic
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Summary:The divergently transcribed sacBK and sacAR operons, which are involved in the utilization of sucrose by Lactococcus lactis NZ9800, were examined by transcriptional and gene inactivation studies. Northern analyses of RNA isolated from cells grown at the expense of different carbon sources revealed three sucrose-inducible transcripts: one of 32 kb containing sacB and sacK, a second of 3.4 kb containing sacA and sacR, and a third of 1.8 kb containing only sacR. The inactivation of the sacR gene by replacement recombination resulted in the constitutive transcription of the sacBK and sacAR operons in the presence of different carbon sources, indicating that SacR acts as a repressor of transcription.
ISSN:0021-9193
1098-5530
DOI:10.1128/jb.181.6.1924-1926.1999