Anti-bacterial and Anti-biofilm Effects of Equol on Yersinia enterocolitica

Yersinia enterocolitica has clinical significance due to its etiological role in yersiniosis and gastroenteritis. This study was designed to assess anti-bacterial and anti-biofilm effects of equol on Y. enterocolitica via phenotypic and genetic analyses. To determine its anti-bacterial activity, min...

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Bibliographic Details
Published in:Indian journal of microbiology 2022-09, Vol.62 (3), p.401-410
Main Authors: Kim, Hye-Rim, Han, Mi-Suk, Eom, Yong-Bin
Format: Article
Language:English
Subjects:
Antibacterial activity
Bacteria
Biofilms
Biomedical and Life Sciences
Cell density
Etiology
Gastroenteritis
Gene expression
Genetic analysis
Identification
Life Sciences
Medical Microbiology
Microbiology
Motility
Original
Original Research Article
Yersinia enterocolitica
Yersiniosis
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Summary:Yersinia enterocolitica has clinical significance due to its etiological role in yersiniosis and gastroenteritis. This study was designed to assess anti-bacterial and anti-biofilm effects of equol on Y. enterocolitica via phenotypic and genetic analyses. To determine its anti-bacterial activity, minimum inhibitory concentrations (MICs) of equol against clinically isolated Y. enterocolitica strains were analyzed. Subsequently, it was confirmed that the sub-MIC 90 value of equol could inhibit biofilm formation and reduce preformed biofilm. Furthermore, it was found that equol could reduce the expression of biofilm-related ( hmsT ) gene in Y. enterocolitica . This study also demonstrated that equol not only reduced levels of bacterial motility, but also decreased the expression of a motility-related ( flhDC ) gene in Y. enterocolitica . XTT [2,3-bis (2-metoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction analysis revealed that equol attenuated cellular metabolic activities in Y. enterocolitica biofilm. Additionally, changes in biomass and cell density in equol-treated biofilms were visualized using a confocal laser scanning microscope. In conclusion, this study suggests that equol is a potential anti-bacterial and anti-biofilm agent to treat Y. enterocolitica .
ISSN:0046-8991
0973-7715
DOI:10.1007/s12088-022-01020-1