The Novel Role of hnRNP UL1 in Human Cell Nucleoli

hnRNP UL1 plays an important role in cell nuclei, where it is recruited to DNA damage sites and is involved in the repair of DNA double-strand breaks. Furthermore, this protein is known as a transcriptional repressor of RNA polymerase II genes. In the present study, we have shown that hnRNP UL1 is a...

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Bibliographic Details
Published in:International journal of biological sciences 2022-01, Vol.18 (13), p.4809-4823
Main Authors: Cichocka, Marlena, Karlik, Anna, Plewka, Patrycja, Gawade, Kishor, Stępień, Agata, Świergiel, Patrycja, Gadgil, Ankur, Raczyńska, Katarzyna Dorota
Format: Article
Language:English
Subjects:
Apoptosis
Biosynthesis
Cancer
Cell cycle
Cell growth
CHK1 protein
Chromosomes
CRISPR
Damage
Deoxyribonucleic acid
DNA
DNA damage
DNA repair
DNA-directed RNA polymerase
Gene expression
Genomes
Kinases
Nucleoli
Proteins
Radiation
Repair
Research Paper
Ribosomal DNA
RNA polymerase
RNA polymerase II
XRCC1 protein
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Summary:hnRNP UL1 plays an important role in cell nuclei, where it is recruited to DNA damage sites and is involved in the repair of DNA double-strand breaks. Furthermore, this protein is known as a transcriptional repressor of RNA polymerase II genes. In the present study, we have shown that hnRNP UL1 is also localized in the nucleoli of human cells. Upon investigating its function, we found that hnRNP UL1 stimulates ribosomal DNA (rDNA) gene transcription. Moreover, we observed that cells with hnRNP UL1 silencing exhibited increased sensitivity to DNA damage. We also showed that hnRNP UL1 interacts with γH2A.X, RPA32, XRCC1, and Chk1 in cell nucleoli, suggesting its involvement in the repair of rDNA damage.
ISSN:1449-2288
1449-2288
DOI:10.7150/ijbs.75084