Growth phase-dependent regulation of Vsr endonuclease may contribute to 5-methylcytosine mutational hot spots in Escherichia coli

Using rabbit polyclonal antibodies, we have shown that the Dcm cytosine methylase of Escherichia coli is maintained at a constant level during cell growth, while Vsr endonuclease levels are growth phase dependent. Decreased production of Vsr relative to Dcm during the log phase may contribute substa...

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Bibliographic Details
Published in:Journal of bacteriology 1999-07, Vol.181 (14), p.4435-4436
Main Authors: Macintyre, G, Pitsikas, P, Cupples, C G
Format: Article
Language:English
Subjects:
5-Methylcytosine
Bacteria
Bacteriology
Cytosine - analogs & derivatives
Cytosine - metabolism
DNA (Cytosine-5-)-Methyltransferases - genetics
DNA (Cytosine-5-)-Methyltransferases - metabolism
Endodeoxyribonucleases - genetics
Endodeoxyribonucleases - metabolism
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - growth & development
Gene Expression Regulation, Bacterial
Genetics and Molecular Biology
Mutation
Proteins
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Summary:Using rabbit polyclonal antibodies, we have shown that the Dcm cytosine methylase of Escherichia coli is maintained at a constant level during cell growth, while Vsr endonuclease levels are growth phase dependent. Decreased production of Vsr relative to Dcm during the log phase may contribute substantially to the mutability of 5-methylcytosine.
ISSN:0021-9193
1098-5530
DOI:10.1128/jb.181.14.4435-4436.1999