LncRNA LIFR‐AS1 overexpression suppressed the progression of serous ovarian carcinoma

• Published in: Journal of clinical laboratory analysis 2022-08, Vol.36 (8), p.e25470-n/a
• Main Authors: Liu, Fang, Cao, Linyan, Zhang, Yufang, Xia, Xinyi, Ji, Yanhua
• Format: Article
• Language: English
• Subjects: Apoptosis; Bioinformatics; Biomarkers; Breast cancer; Cancer therapies; Caspase; Cell proliferation; Colorectal carcinoma; epithelial–mesenchymal transition; Gene amplification; Genomics; Hybridization; LIFR‐AS1; Lymph nodes; Medical prognosis; Mesenchyme; Metastases; Non-coding RNA; Ovarian cancer; Ovarian carcinoma; Patients; Polymerase chain reaction; Prognosis; Proliferating cell nuclear antigen; proliferation; Reagents; Reproductive system; serous ovarian carcinoma; Tumor suppressor genes; Tumors; Wound healing
• ISSN: 0887-8013; 1098-2825
• DOI: 10.1002/jcla.24570

Background Serous ovarian carcinoma (SOC) is a common malignant tumor in female reproductive system. Long noncoding RNA (lncRNA) LIFR‐AS1 is a tumor suppressor gene in colorectal cancer, but its effect and underlying mechanism in SOC are still unclear. Therefore, this study focuses on unveiling the regulatory mechanism of LIFR‐AS1 in SOC. Methods The relationship between LIFR‐AS1 expression and prognosis of SOC patients was analyzed by TCGA database and Starbase, and then, the LIFR‐AS1 expression in SOC tissues and cells was detected by quantitative real‐time PCR (qRT‐PCR) and in situ hybridization (ISH). Besides, the relationship between LIFR‐AS1 and clinical characteristics was analyzed. Also, the effects of LIFR‐AS1 on the biological behaviors of SOC cells were measured by Cell Counting Kit‐8, colony formation, and wound‐healing and Transwell assays, respectively. Western blot and qRT‐PCR were employed to determine the protein expressions of genes related to proliferation (PCNA), apoptosis (cleaved caspase‐3), epithelial‐mesenchymal transition (E‐cadherin, N‐cadherin, and Snail). Results LIFR‐AS1 was lowly expressed in SOC, which was correlated with the poor prognosis of SOC patients. Low expression of LIFR‐AS1 in SOC was associated with the tumor size, clinical stage, lymph node metastasis, and distant metastasis. LIFR‐AS1 overexpression promoted the expressions of cleaved caspase‐3 and E‐cadherin while suppressing the malignant behaviors (proliferation, migration, and invasion) of SOC cells, the expressions of PCNA, N‐cadherin, and Snail. Besides, silencing LIFR‐AS1 exerted the effects opposite to overexpressed LIFR‐AS1. Conclusion LIFR‐AS1 overexpression inhibits biological behaviors of SOC cells, which may be a new therapeutic method. LIFR‐AS1, which is down‐regulated in serous ovarian carcinoma, has the potential to inhibit the progression of serous ovarian carcinoma via regulating the genes related to proliferation, migration, invasion, and apoptosis.