Investigation of the potential of leukoreduction filters in the creation of anti-inflammatory compound

• Published in: Transfusion and apheresis science 2023-02, Vol.62 (1), p.103520, Article 103520
• Main Authors: Balagholi, Sahar, Dabbaghi, Rasul, Mohammadi, Saeed, Abbasi Malati, Zahra, Javan, Mohammad Reza, Eshghi, Pyman
• Format: Article
• Language: English
• Subjects: Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Betamethasone; Betamethasone - pharmacology; COVID-19; Cytokines; Humans; IL1; IL1Ra; Leukoreduction filters; SARA-CoV-2; SARS-CoV-2 - metabolism
• ISSN: 1473-0502; 1878-1683; 1473-0502
• DOI: 10.1016/j.transci.2022.103520

Some viruses such as SARS, SARS-CoV-2, and MERS cause an imbalance in immune responses and leads to an acute inflammatory reaction named cytokine storm. In this situation, an anti-inflammatory component can modulate the immune system and decrease mortality. The aim of this study was investigate the potential of leukoreduction filters (LRFs) in creating an anti-inflammatory compound. In this experimental study, firstly optimal dose of the anti-inflammatory drug was obtained through LRFs treatment with 0.1 mg, 0.4 mg, 0.6 mg of Betamethasone. Then inflammatory and anti-inflammatory cytokine in gene and protein level was evaluated. In the next step, LRFs were categorized into treatment 1, treatment 2, control assay, and control groups and treated with the optimal dose of the drug. Finally, the obtained compound was investigated for the concentration of IL1, IL6, and TNF-α as inflammatory and IL4, IL1Ra, and IL10 as anti-inflammatory cytokines. The results of the current study showed that the concentration of 0.4 mg of Betamethasone lead to a significant increase of anti-inflammatory cytokine in gene and protein levels. The results also showed that the Betamethasone treated groups (treatment1) causes a significant increase in the secretion of anti-inflammatory cytokine compares to the control while inflammatory cytokine remained at the control level. The results showed that under influence of anti-inflammatory drug treatments the production and secretion of anti-inflammatory cytokines can be induced in LRFs.