Identification of a Novel Lipase with AHSMG Pentapeptide in Hypocreales and Glomerellales Filamentous Fungi

Lipases are enzymes that hydrolyze triglycerides to fatty acids and glycerol. A typical element in lipases is a conserved motif of five amino acids (the pentapeptide), most commonly G-X-S-X-G. Lipases with the pentapeptide A-X-S-X-G are present in species of , and the yeast ; they are usually thermo...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2022-08, Vol.23 (16), p.9367
Main Authors: Gutiérrez-Domínguez, Denise Esther, Chí-Manzanero, Bartolomé, Rodríguez-Argüello, María Mercedes, Todd, Jewel Nicole Anna, Islas-Flores, Ignacio, Canseco-Pérez, Miguel Ángel, Canto-Canché, Blondy
Format: Article
Language:English
Subjects:
Amino acid composition
Amino acids
Bacillus - metabolism
Catalysis
Cloning
Conidia
Conserved sequence
Enzymes
Fatty acids
Fungi
Fungi - metabolism
Genomes
Glycerol
Homology
Hypocreales
Hypocreales - metabolism
Lipase
Lipase - metabolism
Localization
Mitochondria
Pathogenicity
Phylogenetics
Phylogeny
Proteins
Pseudomonas - metabolism
Triglycerides
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lipases are enzymes that hydrolyze triglycerides to fatty acids and glycerol. A typical element in lipases is a conserved motif of five amino acids (the pentapeptide), most commonly G-X-S-X-G. Lipases with the pentapeptide A-X-S-X-G are present in species of , and the yeast ; they are usually thermotolerant and solvent resistant. Recently, while searching for true lipases in the genome, one lipase containing the pentapeptide AHSMG was identified. In this study, we cloned from strain B13-1 the lipase ID135964, renamed here as ThaL, which is 97.65% identical with the reference. We found that ThaL is a lid-containing true lipase of cluster III that belongs to a large family comprising highly conserved proteins in filamentous fungi in the orders Hypocreales and Glomerellales, in which predominantly pathogenic fungi are found. ThaL was expressed in conidia, as well as in mycelium, where it was cultured in liquid minimal medium. These results-together with the amino acid composition, absence of a signal peptide, mitochondrial sorting prediction, disordered regions in the protein, and lineage-specific phylogenetic distribution of its homologs-suggest that ThaL is a non-canonical effector. In summary, AHSMG-lipase is a novel lipase family in filamentous fungi, and is probably involved in pathogenicity.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23169367