Insulin Receptor-Related Receptor Regulates the Rate of Early Development in Xenopus laevis

The orphan insulin receptor-related receptor (IRR) encoded by insrr gene is the third member of the insulin receptor family, also including the insulin receptor (IR) and the insulin-like growth factor receptor (IGF-1R). IRR is the extracellular alkaline medium sensor. In mice, insrr is expressed onl...

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Published in:International journal of molecular sciences 2022-08, Vol.23 (16), p.9250
Main Authors: Korotkova, Daria D., Gantsova, Elena A., Goryashchenko, Alexander S., Eroshkin, Fedor M., Serova, Oxana V., Sokolov, Alexey S., Sharko, Fedor, Zhenilo, Svetlana V., Martynova, Natalia Y., Petrenko, Alexander G., Zaraisky, Andrey G., Deyev, Igor E.
Format: Article
Language:English
Subjects:
Bicarbonates
Cell cycle
Embryogenesis
Embryos
Gastrulation
Gene expression
Genes
Growth factors
Insulin
Insulin-like growth factors
Kinases
Ligands
Peptides
Proteins
Siblings
Xenopus laevis
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Summary:The orphan insulin receptor-related receptor (IRR) encoded by insrr gene is the third member of the insulin receptor family, also including the insulin receptor (IR) and the insulin-like growth factor receptor (IGF-1R). IRR is the extracellular alkaline medium sensor. In mice, insrr is expressed only in small populations of cells in specific tissues, which contain extracorporeal liquids of extreme pH. In particular, IRR regulates the metabolic bicarbonate excess in the kidney. In contrast, the role of IRR during Xenopus laevis embryogenesis is unknown, although insrr is highly expressed in frog embryos. Here, we examined the insrr function during the Xenopus laevis early development by the morpholino-induced knockdown. We demonstrated that insrr downregulation leads to development retardation, which can be restored by the incubation of embryos in an alkaline medium. Using bulk RNA-seq of embryos at the middle neurula stage, we showed that insrr downregulation elicited a general shift of expression towards genes specifically expressed before and at the onset of gastrulation. At the same time, alkali treatment partially restored the expression of the neurula-specific genes. Thus, our results demonstrate the critical role of insrr in the regulation of the early development rate in Xenopus laevis.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23169250