Effect of Restraint Stress on Pain Sensitivity, Spinal Trigeminal Nucleus Neurons, and Astrocytes in the Masseter Area of Rats

To explore the changes of pain sensitivity (PS) in the masseter area (MA) in the rat model of psychological stress and the mechanism of action between spinal nucleus neurons and astrocytes in the trigeminal ganglion. The 40 Sprague-Dawley rats were randomly divided into control group (no treatment),...

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Published in:Computational intelligence and neuroscience 2022-08, Vol.2022, p.1-8
Main Author: Han, Feng
Format: Article
Language:English
Subjects:
Adrenocorticotropic hormone
Astrocytes
Body weight
Constraints
Enzymes
Field tests
Glial fibrillary acidic protein
Growth rate
IL-1β
Interleukin 1 receptors
Laboratory animals
Medulla oblongata
Nervous system
Neurons
Open-field behavior
Pain
Pain sensitivity
Proteins
Psychological stress
Rodents
Solitary tract nucleus
Spinal trigeminal nucleus
Stress (Psychology)
Trigeminal ganglion
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description To explore the changes of pain sensitivity (PS) in the masseter area (MA) in the rat model of psychological stress and the mechanism of action between spinal nucleus neurons and astrocytes in the trigeminal ganglion. The 40 Sprague-Dawley rats were randomly divided into control group (no treatment), group A (restraint stress (RS) 1 d), group B (RS 7 d), and group C (RS 14 d). The body weight growth rates (WGR) of rats in each group were compared and the difference of CORT and ACTH in serum was analyzed by ELISA. The open field test and the elevated “cross” maze test were adopted to detect the behavioral changes of rats. Finally, pain threshold of the MA in rats, the activation amount of brain tissue medulla oblongata parts astrocytes markers Glial fibrillary acidic protein (GFAP), and the protein expression of IL-1β and IL-1RI were detected. The results showed the WGR at 7 d and 14 d was greatly lower than control group (P
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The 40 Sprague-Dawley rats were randomly divided into control group (no treatment), group A (restraint stress (RS) 1 d), group B (RS 7 d), and group C (RS 14 d). The body weight growth rates (WGR) of rats in each group were compared and the difference of CORT and ACTH in serum was analyzed by ELISA. The open field test and the elevated “cross” maze test were adopted to detect the behavioral changes of rats. Finally, pain threshold of the MA in rats, the activation amount of brain tissue medulla oblongata parts astrocytes markers Glial fibrillary acidic protein (GFAP), and the protein expression of IL-1β and IL-1RI were detected. The results showed the WGR at 7 d and 14 d was greatly lower than control group (P&lt;0.01). In addition, the activity level and serum CORT and ACTH levels AND mean pain threshold in the MA of groups B and C were greatly lower than control group (P&lt;0.05). The activation rate of GFRP in group C (P&lt;0.01) and the protein expression of IL-1β and IL-1RI (P&lt;0.05) in rat trigeminal ganglion astrocytes of groups B and C was greatly higher than control group, indicating the increase of RS time, the release of IL-1β and IL-1RI can activate neurons and astrocytes in spinal trigeminal nucleus (STN) nerve and increase the PS of the MA.</description><identifier>ISSN: 1687-5265</identifier><identifier>EISSN: 1687-5273</identifier><identifier>DOI: 10.1155/2022/2345039</identifier><identifier>PMID: 36035817</identifier><language>eng</language><publisher>New York: Hindawi</publisher><subject>Adrenocorticotropic hormone ; Astrocytes ; Body weight ; Constraints ; Enzymes ; Field tests ; Glial fibrillary acidic protein ; Growth rate ; IL-1β ; Interleukin 1 receptors ; Laboratory animals ; Medulla oblongata ; Nervous system ; Neurons ; Open-field behavior ; Pain ; Pain sensitivity ; Proteins ; Psychological stress ; Rodents ; Solitary tract nucleus ; Spinal trigeminal nucleus ; Stress (Psychology) ; Trigeminal ganglion</subject><ispartof>Computational intelligence and neuroscience, 2022-08, Vol.2022, p.1-8</ispartof><rights>Copyright © 2022 Feng Han.</rights><rights>COPYRIGHT 2022 John Wiley &amp; Sons, Inc.</rights><rights>Copyright © 2022 Feng Han. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2022 Feng Han. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c410t-4c8875ebdfea40050daffcfc45a2d710a5f98a8e225d10202265fca918c225403</cites><orcidid>0000-0001-5315-2364</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2701964457/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2701964457?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,25753,27924,27925,37012,37013,44590,75126</link.rule.ids></links><search><contributor>Chen, Dong</contributor><contributor>Dong Chen</contributor><creatorcontrib>Han, Feng</creatorcontrib><title>Effect of Restraint Stress on Pain Sensitivity, Spinal Trigeminal Nucleus Neurons, and Astrocytes in the Masseter Area of Rats</title><title>Computational intelligence and neuroscience</title><description>To explore the changes of pain sensitivity (PS) in the masseter area (MA) in the rat model of psychological stress and the mechanism of action between spinal nucleus neurons and astrocytes in the trigeminal ganglion. The 40 Sprague-Dawley rats were randomly divided into control group (no treatment), group A (restraint stress (RS) 1 d), group B (RS 7 d), and group C (RS 14 d). The body weight growth rates (WGR) of rats in each group were compared and the difference of CORT and ACTH in serum was analyzed by ELISA. The open field test and the elevated “cross” maze test were adopted to detect the behavioral changes of rats. Finally, pain threshold of the MA in rats, the activation amount of brain tissue medulla oblongata parts astrocytes markers Glial fibrillary acidic protein (GFAP), and the protein expression of IL-1β and IL-1RI were detected. The results showed the WGR at 7 d and 14 d was greatly lower than control group (P&lt;0.01). In addition, the activity level and serum CORT and ACTH levels AND mean pain threshold in the MA of groups B and C were greatly lower than control group (P&lt;0.05). 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The 40 Sprague-Dawley rats were randomly divided into control group (no treatment), group A (restraint stress (RS) 1 d), group B (RS 7 d), and group C (RS 14 d). The body weight growth rates (WGR) of rats in each group were compared and the difference of CORT and ACTH in serum was analyzed by ELISA. The open field test and the elevated “cross” maze test were adopted to detect the behavioral changes of rats. Finally, pain threshold of the MA in rats, the activation amount of brain tissue medulla oblongata parts astrocytes markers Glial fibrillary acidic protein (GFAP), and the protein expression of IL-1β and IL-1RI were detected. The results showed the WGR at 7 d and 14 d was greatly lower than control group (P&lt;0.01). In addition, the activity level and serum CORT and ACTH levels AND mean pain threshold in the MA of groups B and C were greatly lower than control group (P&lt;0.05). The activation rate of GFRP in group C (P&lt;0.01) and the protein expression of IL-1β and IL-1RI (P&lt;0.05) in rat trigeminal ganglion astrocytes of groups B and C was greatly higher than control group, indicating the increase of RS time, the release of IL-1β and IL-1RI can activate neurons and astrocytes in spinal trigeminal nucleus (STN) nerve and increase the PS of the MA.</abstract><cop>New York</cop><pub>Hindawi</pub><pmid>36035817</pmid><doi>10.1155/2022/2345039</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5315-2364</orcidid><oa>free_for_read</oa></addata></record>
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subjects Adrenocorticotropic hormone
Astrocytes
Body weight
Constraints
Enzymes
Field tests
Glial fibrillary acidic protein
Growth rate
IL-1β
Interleukin 1 receptors
Laboratory animals
Medulla oblongata
Nervous system
Neurons
Open-field behavior
Pain
Pain sensitivity
Proteins
Psychological stress
Rodents
Solitary tract nucleus
Spinal trigeminal nucleus
Stress (Psychology)
Trigeminal ganglion
title Effect of Restraint Stress on Pain Sensitivity, Spinal Trigeminal Nucleus Neurons, and Astrocytes in the Masseter Area of Rats
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