Silver nanoparticle effect on Salmonella enterica isolated from Northern West Egypt food, poultry, and calves

• Published in: Applied microbiology and biotechnology 2022-09, Vol.106 (17), p.5701-5713
• Main Authors: Torky, Helmy Ahmed, Khaliel, Samy Abd-Elsalam, Sedeek, Eman Khalifa, Tawfik, Rasha Gomaa, Bkheet, Ahmad Abo Elmagd, Ebied, Sawsan Khamees, Amin, Heba said, Zahran, Samir Ibrahim, Emara, Hadeer Abd-Elhady, Nofal, Abeer Mohamad, Elghazaly, Eman Moneer
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents; Antibiotic resistance; Antibiotics; Antimicrobial agents; Applied Microbial and Cell Physiology; Biomedical and Life Sciences; Biotechnology; Calves; Cattle; Chickens; Clusters; Drug Resistance, Multiple, Bacterial; Egypt; Electron microscopy; Food; Health aspects; Humans; In vivo methods and tests; Life Sciences; Metal Nanoparticles; Microbial Genetics and Genomics; Microbial Sensitivity Tests; Microbiology; Microscopy; Minimum inhibitory concentration; Multidrug resistance; Nanoparticles; Polymerase chain reaction; Poultry; Salmonella; Salmonella enterica; Salmonella Infections, Animal; Scanning electron microscopy; Silver; Similarity; Strains (organisms); Transmission electron microscopy; Veterinary medicine
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-022-12102-x

A total no. of 65 Salmonella enterica isolates recovered from food samples, feces of diarrheic calves, poultry, and hospital patient in large five cities at Northern West Egypt were obtained from the Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt. The 65  Salmonella enterica isolates had the  inv A gene were grouped into 11  Salmonella enterica serovars with dominance of  S. Enteritidis and S. Kentucky serovars. Their resistance pattern were characterized by using 18 antibiotics from different classes. Approximately 80% of the isolates were multidrug resistant (MDR). Enterobacterial repetitive intergenic consequences polymerase chain reaction (ERIC-PCR) typing of 7 strains of S. Enteritidis showed 5 clusters with dissimilarity 25%. S. Enteritidis clusters in 2 main groups A and B. Group A have 2 human strain (HE2 and HE3) and one food origin (FE7) with a similarity 99%. Group B divided into B1 (FE2) and B2 (FE3) with a similarity ratio ≥ 93%, while ERIC-PCR analysis of 5 strains of S. Kentucky revealed 4 ERIC types, clustered in 2 main groups A and B with similarity 75%. We studied the effect of silver nanoparticles (Ag-NPs) on 10 antibiotic resistant strains of S . Enteritidis and S. Kentucky. The broth microdilution minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were detected. Evaluation of the affection using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed different ratios of Ag-NPs and microorganism as well as at different contact time ended finally with morphological alteration of the bacteria. We submitted new method in vivo to explore the activity of nanosilver in chicken. Key points • Importance of ERIC-PCR to determine the relatedness between Salmonella isolates. • Effect of silver nanoparticles to confront the antibacterial resistance . • Studying the effect of silver nanoparticles in vivo on infected chicken with Salmonella .