Effects of daidzein on antioxidant capacity in weaned pigs and IPEC-J2 cells

• Published in: Animal Nutrition 2022-12, Vol.11, p.48-59
• Main Authors: Li, Yanpin, Jiang, Xianren, Cai, Long, Zhang, Yanli, Ding, Hongbiao, Yin, Jingdong, Li, Xilong
• Format: Article
• Language: English
• Subjects: Antioxidant capacity; Daidzein; Growth performance; IPEC-J2; Original; Oxidative stress; Pig
• ISSN: 2405-6545; 2405-6383
• DOI: 10.1016/j.aninu.2022.06.014

Our previous study found that soybean isoflavones in soybean meal play an important role in improving growth performance and antioxidant capacity in pigs. However, it is still unknown whether long-term supplementation with daidzein, an active molecule deglycosylated from daidzin, in a corn–soybean meal diet can enhance growth performance in pigs. Thus, in the present study, an animal trial was carried out to investigate the effects of dietary supplementation with daidzein on the growth performance and antioxidant capacity of pigs. A total of 80 weaned piglets (40 barrows and 40 females) were assigned to 4 treatments with 5 pens per treatment and 4 piglets per pen and fed a diet supplemented with 0, 25, 50 and 100 mg/kg daidzein for a 72-day trial. In addition, porcine intestinal epithelial cells (IPEC-J2) were used as an in vitro model to explore the underlying antioxidant mechanisms of daidzein. IPEC-J2 cells were treated with 0.6 mM hydrogen peroxide (H 2 O 2 ) in the presence or absence of 40 μM daidzein. The results showed that adding 50 mg/kg of daidzein to the diet significantly improved body weight on day 72, average daily gain (ADG) during days 0 to 72 and plasma superoxide dismutase (SOD) activity on day 42 ( P