In Vitro and In Vivo Investigation on the Effectiveness of Alginate-Based Gastric Mucosal Protective Gel

Objective. To investigate the feasibility and effectiveness of an alginate-based gastric mucosal protective gel on the gastric ulcer. Methods. (1) In the physical protection model, after GES-1 cell attachment add the gel to transwell chamber, add different concentrations of HCl to the gel. Absorbanc...

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Bibliographic Details
Published in:BioMed research international 2022-08, Vol.2022, p.1-11
Main Authors: Zeng, Hang, He, Muye, Yang, Minyi, Meng, Zhu, Wang, Han, Wang, Chunren
Format: Article
Language:English
Subjects:
Alginates
Alginic acid
Cell adhesion
Cell proliferation
Connective tissues
Effectiveness
Endoscopy
Gastric cancer
Gastric juice
Gastric mucosa
Gels (Pharmacy)
Health aspects
Histology
Hydrochloric acid
Hyperplasia
In vivo methods and tests
Infiltration
Inflammation
Laboratory animals
Leukocyte migration
Lymphocytes
Muscles
Necrosis
Prevention
Stomach ulcer
Ulcers
Wound healing
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Summary:Objective. To investigate the feasibility and effectiveness of an alginate-based gastric mucosal protective gel on the gastric ulcer. Methods. (1) In the physical protection model, after GES-1 cell attachment add the gel to transwell chamber, add different concentrations of HCl to the gel. Absorbance was measured to assess proliferation and images of the cells migrating into the wound were taken; then the migration rate of the cells was quantified by comparing images. (2) In the gastric ulcer model, excise the gastric mucosal of SD rats; the gel and fixative were applied on the artificial ulcer immediately. Dissect rats after 10 days, and calculate the wound healing rate and analyzed histology changes. Results. The effect of hydrochloric acid on cells in the lower layer was significantly reduced after the use of gastric mucosal protection gel. The protective gel had an isolation effect on different concentrations of acid. A number of GES-1 were significantly higher than those in the control group at 24 h to 72 h (P
ISSN:2314-6133
2314-6141
DOI:10.1155/2022/8287163