Analysis of double-emulsion droplets with ESI mass spectrometry for monitoring lipase-catalyzed ester hydrolysis at nanoliter scale

Microfluidic double-emulsion droplets allow the realization and study of biphasic chemical processes such as chemical reactions or extractions on the nanoliter scale. Double emulsions of the rare type (o 1 /w/o 2 ) are used here to realize a lipase-catalyzed reaction in the non-polar phase. The surr...

Full description

Saved in:
Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2022-09, Vol.414 (23), p.6977-6987
Main Authors: Heiligenthal, Laura, van der Loh, Marie, Polack, Matthias, Blaha, Maximilian E., Moschütz, Susanne, Keim, Antje, Sträter, Norbert, Belder, Detlev
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Biochemistry
Blood vessels
Characterization and Evaluation of Materials
Chemical composition
Chemical reactions
Chemistry
Chemistry and Materials Science
Double emulsions
Droplets
Emulsification
Emulsions
Food Science
Hydrolysis
Hydrophilicity
Laboratory Medicine
Lipase
Lipophilic
Mass spectrometry
Mass spectroscopy
Methods
Microfluidics
Microreactors
Monitoring/Environmental Analysis
Nitrophenol
p-Nitrophenol
p-Nitrophenyl palmitate
Palmitic acid
Reaction products
Reactors
Research Paper
Scientific imaging
Solvents
Surfactants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Microfluidic double-emulsion droplets allow the realization and study of biphasic chemical processes such as chemical reactions or extractions on the nanoliter scale. Double emulsions of the rare type (o 1 /w/o 2 ) are used here to realize a lipase-catalyzed reaction in the non-polar phase. The surrounding aqueous phase induces the transfer of the hydrophilic product from the core oil phase, allowing on-the-fly MS analysis in single double droplets. A microfluidic two-step emulsification process is developed to generate the (o 1 /w/o 2 ) double-emulsion droplets. In this first example of microfluidic double-emulsion MS coupling, we show in proof-of-concept experiments that the chemical composition of the water layer can be read online using ESI–MS. Double-emulsion droplets were further employed as two-phase micro-reactors for the hydrolysis of the lipophilic ester p- nitrophenyl palmitate catalyzed by the Candida antarctica lipase B (CalB). Finally, the formation of the hydrophilic reaction product p- nitrophenol within the double-emulsion droplet micro-reactors is verified by subjecting the double-emulsion droplets to online ESI–MS analysis. Graphical abstract
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-022-04266-2