Bioactive Vitamin D Attenuates MED28-Mediated Cell Growth and Epithelial–Mesenchymal Transition in Human Colorectal Cancer Cells

Inadequate vitamin D status may increase the risk of developing multiple types of cancer. Epidemiological studies suggest an inverse association between 25-hydroxyvitamin D3 (25(OH)D3) and malignancy, including colorectal cancer. Previous studies have suggested that MED28, a Mediator subunit involve...

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Bibliographic Details
Published in:BioMed research international 2022-08, Vol.2022, p.1-10
Main Authors: Huang, Chun-Yin, Weng, Yu-Ting, Hsieh, Nien-Tsu, Li, Po-Chen, Lee, Tzu-Yi, Li, Chun-I, Liu, Hsiao-Sheng, Lee, Ming-Fen
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Biological activity
Biomarkers
Calciferol
Calcitriol
Cancer
Cancer cells
Care and treatment
Cell culture
Cell differentiation
Cell growth
Cell migration
Colorectal cancer
Colorectal carcinoma
Development and progression
E-cadherin
Epidemiology
Epithelial cells
Gene expression
Gene regulation
Health aspects
Malignancy
Mesenchyme
Metastases
Metastasis
Nutrient interactions
Oncology, Experimental
Physiological aspects
Proteins
Stem cells
Testing
Variance analysis
Vitamin D
Vitamin D3
Vitamin deficiency
Wnt protein
β-Catenin
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Summary:Inadequate vitamin D status may increase the risk of developing multiple types of cancer. Epidemiological studies suggest an inverse association between 25-hydroxyvitamin D3 (25(OH)D3) and malignancy, including colorectal cancer. Previous studies have suggested that MED28, a Mediator subunit involved in transcriptional regulation, is associated with the growth of colorectal cancer cells; however, its role in the progression of metastasis such as epithelial–mesenchymal transition (EMT) and cell migration of colorectal cancer is unclear at present. The aim of this study was to investigate a potentially suppressive effect of calcitriol, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), a bioactive form of vitamin D, and the role of MED28 in the progression of EMT in human colorectal cancer cells. Suppression of MED28 increased the expression of E-cadherin and reduced the expression of several mesenchymal and migration biomarkers and Wnt/β-catenin signaling molecules, whereas overexpression of MED28 enhanced the EMT features. Calcitriol suppressed the expression of MED28, and the effect of calcitriol mirrored that of MED28 silencing. Our data indicate that calcitriol attenuated MED28-mediated cell growth and EMT in human colorectal cancer cells, underlining the significance of MED28 in the progression of colorectal cancer and supporting the potential translational application of calcitriol.
ISSN:2314-6133
2314-6141
DOI:10.1155/2022/2268818