Common methods in mitochondrial research (Review)

Mitochondrial abnormalities are primarily seen in morphology, structure and function. They can cause damage to organs, including the heart, brain and muscle, by various mechanisms, such as oxidative stress, abnormal energy metabolism, or genetic mutations. Identifying and detecting pathophysiologica...

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Bibliographic Details
Published in:International journal of molecular medicine 2022-10, Vol.50 (4), p.1, Article 126
Main Authors: Yin, Yiyuan, Shen, Haitao
Format: Article
Language:English
Subjects:
Apoptosis
Gene mutations
Genetic aspects
Homeostasis
Homogenization
Kinases
Microscopy
Mitochondria
Mitochondrial DNA
Morphology
Physiological aspects
Proteins
Research methodology
Signal transduction
Sucrose
Tumorigenesis
Viscosity
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Summary:Mitochondrial abnormalities are primarily seen in morphology, structure and function. They can cause damage to organs, including the heart, brain and muscle, by various mechanisms, such as oxidative stress, abnormal energy metabolism, or genetic mutations. Identifying and detecting pathophysiological alterations in mitochondria is the principal means of studying mitochondrial abnormalities. The present study reviewed methods in mitochondrial research and focused on three aspects: Mitochondrial extraction and purification, morphology and structure and function. In addition to classical methods, such as electron microscopy and mitochondrial membrane potential monitoring, newly developed methods, such as mitochondrial ultrastructural determination, mtDNA mutation assays, metabolomics and analyses of regulatory mechanisms, have also been utilized in recent years. These approaches enable the accurate detection of mitochondrial abnormalities and provide guidance for the diagnosis and treatment of related diseases.
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.2022.5182