Establishment of a Zebrafish Xenograft Model for in Vivo Investigation of Nasopharyngeal Carcinoma

Nasopharyngeal carcinoma (NPC) is a unique malignant tumor of the head and neck. Despite higher survival rates by the combination of radiotherapy and chemotherapy, the recurrence or metastasis of NPC still occurs at about 10%. Therefore, there is urgent demand to develop more effective in vivo model...

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Bibliographic Details
Published in:Cell transplantation 2022, Vol.31, p.9636897221116085-9636897221116085
Main Authors: Huang, Enyu, Huang, Haofeng, Wu, Longji, Li, Binbin, He, Zhiwei, Zhang, Jingjing
Format: Article
Language:English
Subjects:
Cell proliferation
Chemotherapy
Clinical trials
Danio rerio
Embryos
Metastases
Nasopharyngeal carcinoma
Original
Peptidylprolyl isomerase
Pin1 protein
Radiation therapy
Throat cancer
Tumor markers
Tumors
Xenografts
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Summary:Nasopharyngeal carcinoma (NPC) is a unique malignant tumor of the head and neck. Despite higher survival rates by the combination of radiotherapy and chemotherapy, the recurrence or metastasis of NPC still occurs at about 10%. Therefore, there is urgent demand to develop more effective in vivo models for preclinical trials to investigate the mechanisms of NPC development and progression and to explore better treatment approaches. In this study, we transplanted human NPC CNE1 cells into zebrafish embryos to establish a xenograft model of NPC, where the proliferation and invasion behaviors of NPC cells were investigated in vivo. Combining in vitro and in vivo analyses, we found that activating transcription factor 7 (ATF7) was involved in the occurrence and development of NPC regulated by peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1). The zebrafish NPC xenograft model established here thereby provides an in vivo tool for exploring the occurrence and development of NPC, which may help to identify new tumor markers and develop new therapeutic strategies for the treatment of NPC.
ISSN:0963-6897
1555-3892
DOI:10.1177/09636897221116085