Obtaining and Characterizing Composite Biomaterials of Animal Resources with Potential Applications in Regenerative Medicine

• Published in: Polymers 2022-08, Vol.14 (17), p.3544
• Main Authors: Babeanu, Narcisa, Radu, Nicoleta, Enascuta, Cristina-Emanuela, Alexandrescu, Elvira, Ganciarov, Mihaela, Mohammed, Mohammed Shaymaa Omar, Suica-Bunghez, Ioana Raluca, Senin, Raluca, Ursu, Magdalina, Bostan, Marinela
• Format: Article
• Language: English
• Subjects: Amino acids; Antibiotics; Anticancer properties; Antimicrobial agents; Bacteria; Beef industry; Biocompatibility; Biological products; Biomedical materials; Biopolymers; Cell division; Cells; Cellulose; Chitosan; Collagen; Composite materials; Crustaceans; E coli; Exoskeletons; Fungicides; Gram-positive bacteria; Hydrogels; Hydrogen; Hydrogen bonds; Hydroxyapatite; Microorganisms; Molecular weight; Nanoparticles; Nonsteroidal anti-inflammatory drugs; Peptides; Raw materials; Regenerative medicine; Technology application; Thin films; Toxicity
• ISSN: 2073-4360; 2073-4360
• DOI: 10.3390/polym14173544

Raw materials, such as collagen and chitosan, obtained from by-products from the food industry (beef hides and crustacean exoskeletons), can be used to obtain collagen–chitosan composite biomaterials, with potential applications in regenerative medicine. Functionalization of these composite biomaterials is a possibility, thus, resulting in a molecule with potential applications in regenerative medicine, namely clotrimazole (a molecule with antibacterial, antifungal, and antitumor activity), at a mass ratio (collagen–chitosan–clotrimazole) of 1:1:0.1. This functionalized composite biomaterial has great potential for application in regenerative medicine, due to the following properties: (1) it is porous, and the pores formed are interconnected, due to the use of a mass ratio between collagen and chitosan of 1:1; (2) the size of the formed pores is between 500–50 μm; (3) between collagen and chitosan, hydrogen bonds are formed, which ensure the unity of composite biomaterial; (4) the functionalized bio-composite exhibits in vitro antimicrobial activity for Candida albicans, Staphylococcus aureus, and Staphylococcus aureus MRSA; for the latter microorganism, the antimicrobial activity is equivalent to that of the antibiotic Minocycline; (5) the proliferation tests performed on a standardized line of normal human cells with simple or composite materials obtained by lyophilization do not show cytotoxicity in the concentration range studied (10–500) μg/mL.