Genome Characteristic of Bordetella parapertussis Isolated from Iran

Pertussis also known as whooping cough is a respiratory infection in humans particularly with severe symptoms in infants and usually caused by Bordetella pertussis . However, Bordetella parapertussis can also cause a similar clinical syndrome. During 2012 to 2015, from nasal swabs sent from differen...

Full description

Saved in:
Bibliographic Details
Published in:Current microbiology 2022-10, Vol.79 (10), p.314-314, Article 314
Main Authors: Safarchi, Azadeh, Saedi, Samaneh, Tay, Chin Yen, Lamichhane, Binit, Nakhost Lotfi, Masoumeh, Shahcheraghi, Fereshteh
Format: Article
Language:English
Subjects:
Biochemical tests
Biomedical and Life Sciences
Biotechnology
Bordetella parapertussis
Gene expression
Genomes
Infants
Life Sciences
Microbiology
Nucleotides
Pasture
Pertussis
Phylogeny
Short Communication
Single-nucleotide polymorphism
Virulence factors
Whooping cough
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pertussis also known as whooping cough is a respiratory infection in humans particularly with severe symptoms in infants and usually caused by Bordetella pertussis . However, Bordetella parapertussis can also cause a similar clinical syndrome. During 2012 to 2015, from nasal swabs sent from different provinces to the pertussis reference laboratory of Pasture Institute of Iran for pertussis confirmation, seven B. parapertussis isolates were identified by bacterial culture, biochemical tests, and the presence of IS 1001 insertion in the genome. The expression of pertactin ( Prn ) as one the major virulence factor for bacterial adhesion was investigated using western blot. Moreover, the genomic characteristic of one recently collected isolate, IRBP134, from a seven-month infant was investigated using Illumina NextSeq sequencing protocol. The results revealed the genome with G+C content 65% and genome size 4.7 Mbp. A total of 81 single nucleotide polymorphisms and 13 short insertions and deletions were found in the genome compared to the B. parapertussis 12822 as a reference genome showing ongoing evolutionary changes. A phylogeny relationship of IRBP134 was also investigated using global B. parapertussis available genomes.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-022-03009-x