Astragalus membranaceus and Salvia miltiorrhiza Ameliorate Hypertensive Renal Damage through lncRNA-mRNA Coexpression Network

• Published in: BioMed research international 2022-09, Vol.2022, p.3002353-14
• Main Authors: Zhou, Le, Han, Cong, Liu, Yao, Cui, Tao, Shen, Zhen, Li, Xiang-yu, Jiang, Yue-hua, Li, Wei
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Astragalus (Plants); Astragalus membranaceus; Astragalus propinquus; Blood pressure; Care and treatment; Chemical properties; Chinese medicine; Damage; Fatty Acids; Gene expression; Gene sequencing; Genes; Health aspects; Hypertension; Hypertension - drug therapy; Hypertension - genetics; Interleukin 6; Kidney - metabolism; Kinases; Laboratory animals; Medicine, Chinese; mRNA; Non-coding RNA; Oxidation; Oxidative stress; Pathology; Peroxisome Proliferator-Activated Receptors; Pharmacology, Experimental; Rats; Rats, Inbred SHR; Renal function; Renal hypertension; RNA; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sage; Salvia miltiorrhiza; Signal transduction; Signaling; Urine
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2022/3002353

lncRNAs and mRNA are closely associated with hypertensive renal damage, and Astragalus membranaceus and Salvia miltiorrhiza (AS) have a therapeutic effect; however, the mechanism of AS to ameliorate hypertensive renal damage through the co-expression network of lncRNA-mRNA was unclear. In this study, we investigated the role of AS regulated the coexpression network of lncRNA-mRNA in improving hypertensive renal damage. Sixteen 24-week old spontaneous hypertensive rats (SHRs) were randomly divided into model group (M) and drug intervention group (AS, 5.9 g/kg), 8 Wistar Kyoto rats (WKY) of the same age as normal group (N). The treatment of rats was 4 weeks. Detecting the change of blood pressure, renal pathology and renal function related indicators, and lncRNA and mRNA sequencing and joint analysis was performed on the kidney. AS reduced blood pressure; decreased urine NAG, urine mALB, serum CysC, and IL-6; and improved renal pathology compared with group M. Simultaneously, AS reversed the disordered expression of 178 differential expression (DE) mRNAs and 237 DE-lncRNAs in SHRs, and their joint analysis showed that 13 DE-mRNAs and 32 DE-lncRNAs were coexpressed. Further analysis of 13 coexpressed DE-mRNAs showed negative regulation of blood pressure and fatty acid beta-oxidation was highly enriched in GO pathways, PPAR signaling pathway was highly enriched in KEGG pathways, and the verification related to these pathways was also highly consistent with the sequence. AS can alleviate hypertensive renal damage through the coexpression network of lncRNA-mRNA, of which coexpressed 13 DE-mRNAs and 32 DE-lncRNAs were the important targets, and the pathway negative regulation of blood pressure, fatty acid beta-oxidation, and PPAR signaling pathway play a major regulatory role.