TIPE2 protects cardiomyocytes from ischemia‑reperfusion‑induced apoptosis by decreasing cell autophagy via the mTORC1 signaling pathway

In cardiac ischemia-reperfusion (I/R), autophagy of hyperactivated cardiomyocytes degrades normal proteins and organelles, destroys cells and causes irreversible cell death. The present study aimed to determine the molecular mechanism through which TNF-[alpha]-induced protein 8-like protein 2 (TIPE2...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2022-10, Vol.24 (4), Article 613
Main Authors: Cheng, Gong, Huang, Xiaoyan, You, Penghua, Feng, Panpan, Jia, Shuo, Zhang, Ji, You, Hongjun, Chang, Fengjun
Format: Article
Language:English
Subjects:
Apoptosis
Autophagy
Autophagy (Cytology)
Cardiomyocytes
Cardiovascular disease
Cell culture
Cellular signal transduction
Coronary vessels
Electrocardiography
Health aspects
Heart attacks
Heart cells
Inflammation
Ischemia
Plasmids
Prevention
Proteins
Reperfusion (Physiology)
Statistical analysis
Vein & artery diseases
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Summary:In cardiac ischemia-reperfusion (I/R), autophagy of hyperactivated cardiomyocytes degrades normal proteins and organelles, destroys cells and causes irreversible cell death. The present study aimed to determine the molecular mechanism through which TNF-[alpha]-induced protein 8-like protein 2 (TIPE2) regulates cardiomyocyte apoptosis via autophagy in I/R. The results revealed that the number of apoptotic cells and the protein expression levels of TIPE2 in the heart tissue of I/R model mice were significantly increased. In vitro, the overexpression of TIPE2 decreased oxygen glucose deprivation (OGD)-induced autophagy, apoptosis and activation of the mTOR complex 1 (mTORC1) signaling pathway in H9c2 cells. Treatment with the mTORC1 inhibitor not only inhibited the TIPE2-activated mTORC1 signaling pathway, but also increased OGD-induced autophagy and apoptosis of H9c2 cells. In conclusion, the results of the present study revealed that TIPE2 may protect cardiomyocytes from I/R-induced apoptosis by decreasing cell autophagy via the mTORC1 signaling pathway. Key words: TNF-[alpha]-induced protein 8-like protein 2, mTOR complex 1, autophagy
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2022.11550