Increasing the Grain Yield and Grain Protein Content of Common Wheat ( Triticum aestivum ) by Introducing Missense Mutations in the Q Gene

• Published in: International journal of molecular sciences 2022-09, Vol.23 (18), p.10772
• Main Authors: Chen, Qing, Guo, Zhenru, Shi, Xiaoli, Wei, Meiqiao, Fan, Yazhen, Zhu, Jing, Zheng, Ting, Wang, Yan, Kong, Li, Deng, Mei, Cao, Xinyou, Wang, Jirui, Wei, Yuming, Jiang, Qiantao, Jiang, Yunfeng, Chen, Guoyue, Zheng, Youliang, Qi, Pengfei
• Format: Article
• Language: English
• Subjects: Alleles; Binding sites; Crop yield; Domains; Domestication; Edible Grain - chemistry; Edible Grain - genetics; Ethyl methanesulfonate; Ethyl Methanesulfonate - metabolism; Fertilizers; Food quality; Genes, vif; Germplasm; Grain; Grain Proteins - metabolism; miRNA; Missense mutation; Mutagenesis; Mutation; Mutation, Missense; Nitrogen; Nutrient content; Phenotype; Plant Breeding; Proteins; Quantitative Trait Loci; Transcription factors; Triticum - genetics; Triticum - metabolism; Triticum aestivum; Wheat
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms231810772

Grain yield (GY) and grain protein content (GPC) are important traits for wheat breeding and production; however, they are usually negatively correlated. The gene is the most important domestication gene in cultivated wheat because it influences many traits, including GY and GPC. Allelic variations in the gene may positively affect both GY and GPC. Accordingly, we characterized two new alleles ( and ) obtained through ethyl methanesulfonate-induced mutagenesis. Compared with the wild-type allele, contains a missense mutation in the sequence encoding the first AP domain, whereas has two missense mutations: one in the sequence encoding the second AP domain and the other in the microRNA172-binding site. The allele did not significantly affect GPC or other processing quality parameters, but it adversely affected GY by decreasing the thousand kernel weight and grain number per spike. In contrast, positively affected GPC and GY by increasing the thousand kernel weight and grain number per spike. Thus, we generated novel germplasm relevant for wheat breeding. A specific molecular marker was developed to facilitate the use of the allele in breeding. Furthermore, our findings provide useful new information for enhancing cereal crops via non-transgenic approaches.