Acute and inherited piRNA-mediated silencing in a rde-3 ribonucleotidyltransferase mutant

We recently developed a piRNA-based silencing assay (piRNAi) to study small-RNA mediated epigenetic silencing: acute gene silencing is induced by synthetic piRNAs expressed from extra-chromosomal array and transgenerational inheritance can be quantified after array loss. The assay allows inheritance...

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Bibliographic Details
Published in:microPublication biology 2022-01, Vol.2022
Main Authors: Priyadarshini, Monika, AlHarbi, Sarah, Frøkjær-Jensen, Christian
Format: Article
Language:English
Subjects:
C. Elegans
New Finding
Phenotype Data
Online Access:Get full text
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Summary:We recently developed a piRNA-based silencing assay (piRNAi) to study small-RNA mediated epigenetic silencing: acute gene silencing is induced by synthetic piRNAs expressed from extra-chromosomal array and transgenerational inheritance can be quantified after array loss. The assay allows inheritance assays by injecting piRNAs directly into mutant animals and targeting endogenous genes ( e.g. , him-5 and him-8 ) with obvious phenotypes (increased male frequency). Here we demonstrate the piRNAi assay by quantifying acute and inherited silencing in the ribonucleotidyltransferase rde-3 (ne3370) mutant. In the absence of rde-3, acute silencing was reduced but still detectable, whereas inherited silencing was abolished.
ISSN:2578-9430
DOI:10.17912/micropub.biology.000638