Shoutai Wan Improves Embryo Survival by Regulating Aerobic Glycolysis of Trophoblast Cells in a Mouse Model of Recurrent Spontaneous Abortion

Background. During embryo implantation, the blastocyst exhibits a high capacity for aerobic glycolysis, which results in a unique microenvironment of high lactate/low pH at the maternal-fetal interface. Shoutai Wan (STW) is an effective Chinese herbal formula widely used in the clinical treatment of...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2022-09, Vol.2022, p.1-17
Main Authors: Liang, Xiao, Tang, Siling, Li, Dandan, Song, Yajing, He, Ming, Duan, Yancang, Du, Huilan
Format: Article
Language:English
Subjects:
Abortion
Aerobic capacity
Bioengineering
Chorionic gonadotropin
Cytology
Dehydrogenases
Drug dosages
Embryos
Enzymes
Etiology
Fetuses
Gene expression
Glycolysis
Gonadotropins
Herbal medicine
Implantation
Kidneys
Kinases
Laboratory animals
Lactic acid
Medicine
Microenvironments
Miscarriage
Pituitary (anterior)
Pregnancy
Progesterone
Rodents
Uterus
Vagina
Women
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Summary:Background. During embryo implantation, the blastocyst exhibits a high capacity for aerobic glycolysis, which results in a unique microenvironment of high lactate/low pH at the maternal-fetal interface. Shoutai Wan (STW) is an effective Chinese herbal formula widely used in the clinical treatment of recurrent spontaneous abortion (RSA). However, the specific molecular mechanism by which STW prevents abortion is yet to be elucidated. Methods. Female CBA/J mice were allocated into six groups randomly and then mated with BALB/c mice as the control group, DBA/2 mice as the RSA model, CBA/J×DBA/2 mice treated with dydrogesterone as the DQYT group, or CBA/J×DBA/2 mice treated with low, medium, and high-dose STW as the STW-L, STW-M, and STW-H groups, respectively. Drug administration started 14 days before mating and ended on the 14th day of pregnancy. The embryo loss rate of each group was calculated on day 14 of gestation, and the pregnancy outcomes of the mice in each group were observed. The mouse serum was collected to determine the levels of progesterone (P) and chorionic gonadotropin (CG). The activities of HK2, PKM2, and LDHA, the key glycolytic enzymes in each group, were detected. The expressions of lactate, ATP, HK2, PKM2, LDHA, MCT4, GLUT1, and GPR81 as well as the morphology of trophoblast cells were examined. Results. The embryo loss rate and adverse pregnancy outcomes were significantly increased (P
ISSN:1741-427X
1741-4288
DOI:10.1155/2022/8251503