Measurement of glucose metabolism in the occipital lobe and frontal cortex after oral administration of [1-13C]glucose at 9.4 T

• Published in: Journal of cerebral blood flow and metabolism 2022-10, Vol.42 (10), p.1890-1904
• Main Authors: Ziegs, Theresia, Dorst, Johanna, Ruhm, Loreen, Avdievitch, Nikolai, Henning, Anke
• Format: Article
• Language: English
• Subjects: Administration, Oral; Amino Acids; Aspartic Acid - metabolism; Brain - metabolism; Carbon Isotopes - metabolism; Frontal Lobe - diagnostic imaging; Frontal Lobe - metabolism; Glucose - metabolism; Glutamic Acid - metabolism; Glutamine - metabolism; Humans; Magnetic Resonance Spectroscopy - methods; Occipital Lobe - diagnostic imaging; Occipital Lobe - metabolism; Original
• ISSN: 0271-678X; 1559-7016
• DOI: 10.1177/0271678X221104540

For the first time, labeling effects after oral intake of [1-13C]glucose are observed in the human brain with pure 1H detection at 9.4 T. Spectral time series were acquired using a short-TE 1H MRS MC-semiLASER (Metabolite Cycling semi Localization by Adiabatic SElective Refocusing) sequence in two voxels of 5.4 mL in the frontal cortex and the occipital lobe. High-quality time-courses of [4-13C]glutamate, [4-13C]glutamine, [3-13C]glutamate + glutamine, [2-13C] glutamate+glutamine and [3-13C]aspartate for individual volunteers and additionally, group-averaged time-courses of labeled and non-labeled brain glucose could be obtained. Using a one-compartment model, mean metabolic rates were calculated for each voxel position: The mean rate of the TCA-cycle (Vtca) value was determined to be 1.36 and 0.93 μmol min−1 g−1, the mean rate of glutamine synthesis (Vgln) was calculated to be 0.23 and 0.45 μmol min−1 g−1, the mean exchange rate between cytosolic amino acids and mitochondrial Krebs cycle intermediates (Vx) rate was found to be 0.57 and 1.21 μmol min−1 g−1 for the occipital lobe and the frontal cortex, respectively. These values were in agreement with previously reported data. Altogether, it can be shown that this most simple technique combining oral administration of [1-13C]Glc with pure 1H MRS acquisition is suitable to measure metabolic rates.