Developmental changes in ciliary composition during gametogenesis in Chlamydomonas

transitions from mitotically dividing vegetative cells to sexually competent gametes of two distinct mating types following nutrient deprivation. Gametes of opposite mating type interact via their cilia, initiating an intraciliary signaling cascade and ultimately fuse forming diploid zygotes. The pr...

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Bibliographic Details
Published in:Molecular biology of the cell 2022-06, Vol.33 (7), p.br10-br10
Main Authors: Sakato-Antoku, Miho, King, Stephen M
Format: Article
Language:English
Subjects:
Animals
Brief Report
Chlamydomonas - metabolism
Chlamydomonas reinhardtii - metabolism
Cilia - metabolism
Gametogenesis
Life Cycle Stages
Proteomics
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Summary:transitions from mitotically dividing vegetative cells to sexually competent gametes of two distinct mating types following nutrient deprivation. Gametes of opposite mating type interact via their cilia, initiating an intraciliary signaling cascade and ultimately fuse forming diploid zygotes. The process of gametogenesis is genetically encode, and a previous study revealed numerous significant changes in mRNA abundance during this life-cycle transition. Here we describe a proteomic analysis of cilia derived from vegetative and gametic cells of both mating types in an effort to assess the global changes that occur within the organelle during this process. We identify numerous membrane- and/or matrix-associated proteins in gametic cilia that were not detected in cilia from vegetative cells. This includes the pro-protein from which the GATI-amide gametic chemotactic modulator derives, as well as receptors, a dynamin-related protein, ammonium transporters, two proteins potentially involved in the intraciliary signaling cascade-driven increase in cAMP, and multiple proteins with a variety of interaction domains. These changes in ciliary composition likely directly affect the functional properties of this organelle as the cell transitions between life-cycle stages.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.E22-02-0033