Development of CD163 receptor-based enzyme-linked immunosorbent assay for diagnosis of porcine reproductive and respiratory syndrome virus

Porcine reproductive and respiratory syndrome (PRRS) is an important economical disease in the global swine industry. The accurate detection of the PRRS virus (PRRSV) antigen is essential for the disease control and prevention programme. In this study, an indirect enzyme-linked immunosorbent test (P...

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Bibliographic Details
Published in:3 Biotech 2022-11, Vol.12 (11), p.325-325, Article 325
Main Authors: Deb, Rajib, Yadav, Ajay Kumar, Sengar, Gyanendra Singh, Sonowal, Joyshikh, Lalita, D., Pegu, Seema Rani, Singh, Indra, Linda, Ningthoukhongjam, Das, Pranab Jyoti, Kumar, Satish, Pal, Prasanna, Paul, Souvik, Rajkhowa, Swaraj, Gupta, Vivek Kumar
Format: Article
Language:English
Subjects:
Agriculture
Animal diseases
Antigens
Assaying
Betaarterivirus suid 1
Bioinformatics
Biomaterials
Biotechnology
Cancer Research
CD163 antigen
Chemistry
Chemistry and Materials Science
diagnostic techniques
Disease control
Enzyme-linked immunosorbent assay
Enzymes
ligands
porcine reproductive and respiratory syndrome
pork industry
Protocols and Methods
Receptors
Respiratory diseases
Stem Cells
Swine
Viral diseases
Viruses
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Summary:Porcine reproductive and respiratory syndrome (PRRS) is an important economical disease in the global swine industry. The accurate detection of the PRRS virus (PRRSV) antigen is essential for the disease control and prevention programme. In this study, an indirect enzyme-linked immunosorbent test (PRRSVCD163-iELISA) was developed for the detection of the PRRSV antigen in samples of post-mortem swine tissue using the recombinant pig CD163 receptor protein as the capture ligand. The test was found to be specific for PRRSV, with no cross-reactions with other prevalent pig viral pathogens. The assay was validated by testing 217 post-mortem porcine tissue samples and the results were found to be satisfactory with a relative accuracy of 88.88%. Our assay is also quite precise, with intra- and inter-assay CVs of 6% and 10%, respectively. These findings imply that the PRRSVCD163-iELISA developed is capable of detecting the PRRSV antigen in swine post-mortem tissue samples. This research showed that porcine CD163, the PRRSV cellular receptor, can be exploited to build a diagnostic technique for the detection of PRRSV antigen.
ISSN:2190-572X
2190-5738
2190-5738
DOI:10.1007/s13205-022-03376-z