Wound swabs versus biopsies to detect methicillin resistant Staphylococcus aureus in experimental equine wounds

Objective To compare: (1) the load and diversity of cultivatable bacterial species isolated from tissue biopsies with cultures from surface swabs, and (2) the ability of each technique to detect methicillin‐resistant Staphylococcus aureus (MRSA) in a model of MRSA‐infected equine wounds. Study desig...

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Bibliographic Details
Published in:Veterinary surgery 2022-11, Vol.51 (8), p.1196-1205
Main Authors: Brock, Abbi K., Chamoun‐Emanuelli, Ana M., Howard, Emily A., Huntzinger, Katie D., Lawhon, Sara D., Bryan, Laura K., Cosgriff‐Hernandez, Elizabeth M., Cohen, Noah D., Whitfield‐Cargile, Canaan M.
Format: Article
Language:English
Subjects:
Bacteria
Biopsy
Drug resistance
Horses
In vivo methods and tests
Inoculation
Methicillin
Pure culture
Sampling
Sampling methods
Sampling techniques
Species diversity
Staphylococcus aureus
Staphylococcus infections
Tissues
Wounds
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Summary:Objective To compare: (1) the load and diversity of cultivatable bacterial species isolated from tissue biopsies with cultures from surface swabs, and (2) the ability of each technique to detect methicillin‐resistant Staphylococcus aureus (MRSA) in a model of MRSA‐infected equine wounds. Study design Experimental in vivo study. Animals Three light‐breed adult horses. Methods Four 2.5 × 2.5 cm full‐thickness skin wounds were created on the dorsolateral aspect of each forelimb. Five days later, each wound was inoculated with a pure culture of MRSA (ATCC 43300). One hundred microlitres of 0, 5 × 108, 5 × 109 or 5 × 1010 colony forming units (CFU)/ml was used to inoculate each wound. Surface swabs (Levine technique) and tissue biopsy samples (3 mm punch biopsy) were obtained at 2, 7, 14, and 21 days after inoculation. Quantitative aerobic culture was performed using routine clinical techniques. Results A similar bacterial profile was identified from the culture of each wound‐sampling technique and there was moderate correlation (R = 0.49, P 
ISSN:0161-3499
1532-950X
DOI:10.1111/vsu.13872