Oxicam-type nonsteroidal anti-inflammatory drugs enhance Agrobacterium-mediated transient transformation in plants

Agrobacterium-mediated transformation is a key innovation for plant breeding, and routinely used in basic researches and applied biology. However, the transformation efficiency is often the limiting factor of this technique. In this study, we discovered that oxicam-type nonsteroidal anti-inflammator...

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Published in:Plant Biotechnology 2022/09/25, Vol.39(3), pp.323-327
Main Authors: Choi, Seung-won, Kumaishi, Kie, Motohashi, Reiko, Enoki, Harumi, Chacuttayapong, Wiluk, Takamizo, Tadashi, Saika, Hiroaki, Endo, Masaki, Yamada, Tetsuya, Hirose, Aya, Koizuka, Nobuya, Kimura, Seisuke, Kawakatsu, Yaichi, Koga, Hiroyuki, Ito, Emi, Shirasu, Ken, Ichihashi, Yasunori
Format: Article
Language:English
Subjects:
Agrobacterium
Agrobacterium-mediated transient transformation
Anti-inflammatory agents
Arabidopsis thaliana
Efficiency
Fluorescence
Genetic transformation
Immune system
Immunosuppressive agents
Inflammation
Jasmonic acid
Jatropha curcas
Leaves
Localization
Nonsteroidal anti-inflammatory drugs
Plant bacterial diseases
Plant breeding
Plant species
Plants (botany)
Salicylic acid
tenoxicam
Transformations
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Summary:Agrobacterium-mediated transformation is a key innovation for plant breeding, and routinely used in basic researches and applied biology. However, the transformation efficiency is often the limiting factor of this technique. In this study, we discovered that oxicam-type nonsteroidal anti-inflammatory drugs, including tenoxicam (TNX), increase the efficiency of Agrobacterium-mediated transient transformation. TNX treatment increased the transformation efficiency of Agrobacterium-mediated transformation of Arabidopsis thaliana mature leaves by agroinfiltration. The increase of efficiency by TNX treatment was not observed in dde2/ein2/pad4/sid2 quadruple mutant, indicating that TNX inhibits the immune system mediated by jasmonic acid, ethylene, and salicylic acid against to Agrobacterium. We also found that TNX-treatment is applicable for the transient expression and subcellular localization analysis of fluorescent-tagged proteins in Arabidopsis leaf cells. In addition, we found that TNX increases the efficiency of Agrobacterium-mediated transient transformation of Jatropha. Given that treatment with oxicam compounds is a simple and cost effective method, our findings will provide a new option to overcome limitations associated with Agrobacterium-mediated transformation of various plant species.
ISSN:1342-4580
1347-6114
DOI:10.5511/plantbiotechnology.22.0312a