Protocol to assess human germinal center responses to SARS-CoV-2 vaccination using a combined fine needle aspiration and spectral flow cytometry approach

SARS-CoV-2 mRNA vaccines drive the generation of affinity-matured B cell responses through germinal center (GC) reactions in vaccine draining lymph nodes. Herein, we describe a procedure for the acquisition of human lymph node samples via an ultrasound-guided fine needle aspiration based-approach. A...

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Bibliographic Details
Published in:STAR protocols 2022-10
Main Authors: Bettini, Emily, Lederer, Katlyn, Sharpe, Hannah, Kaminski, Mary, Jones, Lisa, Locci, Michela
Format: Article
Language:English
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Protocol
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Summary:SARS-CoV-2 mRNA vaccines drive the generation of affinity-matured B cell responses through germinal center (GC) reactions in vaccine draining lymph nodes. Herein, we describe a procedure for the acquisition of human lymph node samples via an ultrasound-guided fine needle aspiration based-approach. Additionally, we outline a suggested approach for the analysis of CD4 T helper cell subsets as well as antigen-specific GC B cells, memory B cells, and plasmablasts by high-parameter, spectral flow cytometry. Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667
DOI:10.1016/j.xpro.2022.101840