Extraction, Characterization, and Platelet Inhibitory Effects of Two Polysaccharides from the Cs-4 Fungus

Cardiovascular diseases are associated with platelet hyperactivity, and downregulating platelet activation is one of the promising antithrombotic strategies. This study newly extracted two polysaccharides (purified exopolysaccharides, EPSp and purified intercellular exopolysaccharides, IPSp) from Co...

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Published in:International journal of molecular sciences 2022-10, Vol.23 (20), p.12608
Main Authors: Mao, Yu-Heng, Song, Feng-Lin, Xu, Yi-Xuan, Song, Ang-Xin, Wang, Zhao-Mei, Zhao, Ming-Zhu, He, Fang, Tian, Ze-Zhong, Yang, Yan
Format: Article
Language:English
Subjects:
Arabinose
Binding sites
Cardiovascular diseases
Chromatography
Excitatory postsynaptic potentials
Exopolysaccharides
Fatty acids
Feces
Fermentation
Fourier transforms
Fungi
Galactose
Hyperactivity
Inhibitory postsynaptic potentials
Mannose
Metabolites
Mycelia
Polysaccharides
Proteins
Thrombosis
Variance analysis
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Summary:Cardiovascular diseases are associated with platelet hyperactivity, and downregulating platelet activation is one of the promising antithrombotic strategies. This study newly extracted two polysaccharides (purified exopolysaccharides, EPSp and purified intercellular exopolysaccharides, IPSp) from Cordyceps sinensis Cs-4 mycelial fermentation powder, and investigated the effects of the two polysaccharides and their gut bacterial metabolites on platelet functions and thrombus formation. EPSp and IPSp are majorly composed of galactose, mannose, glucose, and arabinose. Both EPSp and IPSp mainly contain 4-Galp and 4-Glcp glycosidic linkages. EPSp and IPSp significantly inhibited human platelet activation and aggregation with a dose-dependent manner, and attenuated thrombus formation in mice without increasing bleeding risk. Furthermore, the EPSp and IPSp after fecal fermentation showed enhanced platelet inhibitory effects. The results have demonstrated the potential value of Cs-4 polysaccharides as novel protective ingredients for cardiovascular diseases.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232012608