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Suppression of the Testis-Specific Transcription of the ZBTB32 and ZNF473 Genes in Germ Cell Tumors
The family of genes containing C2H2 zinc finger domains, which has more than 700 members, is one of the largest in the genome. Of particular interest are C2H2 genes with potential tissue-specific transcription, which determine the functional properties of individual cell types, including those assoc...
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Published in: | Actanaturae 2022-07, Vol.14 (3), p.85-94 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Online Access: | Get full text |
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Summary: | The family of genes containing C2H2 zinc finger domains, which has more than
700 members, is one of the largest in the genome. Of particular interest are
C2H2 genes with potential tissue-specific transcription, which determine the
functional properties of individual cell types, including those associated with
pathological processes. The aim of this work was to identify C2H2 family genes
with tissue-specific transcription and analyze changes in their activity during
tumor progression. To search for these genes, we used four databases containing
data on gene transcription in human tissues obtained by RNA-Seq analysis. The
analysis showed that, although the major part of the C2H2 family genes is
transcribed in virtually all tissues, a group of genes has tissue-specific
transcription, with most of the transcripts being found in the testis. After
having compared all four databases, we identified nine such genes. The
testis-specific transcription was confirmed for two of them, namely ZBTB32 and
ZNF473, using quantitative PCR of cDNA samples from different organs. A
decrease in ZBTB32 and ZNF473 transcription levels was demonstrated in germ
cell tumors. The studied genes can serve as candidate markers in germ cell
tumors. |
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ISSN: | 2075-8251 |
DOI: | 10.32607/actanaturae.11620 |